文献类型：期刊文献

英文题名：Synthesis and Characterization of Folic Acid Labeled Upconversion Fluorescent Nanoprobes for in vitro Cancer Cells Targeted Imaging

作者：Huang, Ming[1];Wang, Lijun[2];Zhang, Xiaojuan[1];Zhou, Jin[1];Liu, Lihua[1];Pan, Yuefang[1];Yu, Bin[1];Yu, Zhangsen[1]

机构：[1]Shaoxing Univ, Sch Med, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Sch Chem & Chem Engn, Shaoxing 312000, Peoples R China

年份：2017

卷号：12

期号：5

外文期刊名：NANO

收录：SCI-EXPANDED(收录号：WOS:000402317800006)、、WOS

基金：We are grateful for the financial support from the College Students' Science and Technology Innovation Project of Zhejiang Province, China (No. 2014R426024), National Natural Science Foundation of China (51402195), Educational Commission of Zhejiang Province, China (No. Y201225801), Shaoxing University Research Project (No. 2011LG1018) and Shaoxing City Science and Technology Bureau Project (No. 2013B70062).

语种：英文

外文关键词：Upconversion nanoparticles; NaLuF4:Yb,Er/PEI-FA; targeting fluorescent cell imaging

外文摘要：Upconversion nanoparticles (UCNPs) are widely used in the field of biomedicine, such as biosensing, cell labeling and medical multimodal imaging because of their unique optical properties. In this paper, we demonstrated the synthesis of polyethylenimine-modified NaLuF4:Yb, Er (RE = Lu-0:78, Yb-0.18, Er-0.02) UCNPs in three different solvents, such as water, ethylene glycol and diethylene glycol. The as-prepared UCNPs were characterized and the experimental results showed that the UCNPs synthesized in ethylene glycol had excellent properties. The obtained UCNPs in ethylene glycol had the smallest particle size and uniform size distribution, and the pure cubic phase of crystallization and Dynamic light scattering and particle dispersion index (DLS/Pdi) were the smallest. What's more, the upconversion fluorescence intensity was 7 and 52 times greater than that of UCNPs synthesized in diethylene glycol and water, respectively. In addition, the factors of reaction solvent that had an impact on the particle size, morphology, crystalline phase, DLS and upconversion fluorescence intensity of the synthesized UCNPs were discussed. Moreover, in order to obtain the targeted nanoprobe, we used an EDC/NHS covalent coupling method to modify folic acid to the NaLuF4: Yb, Er/PEI UCNP surface. The NaLuF4: Yb, Er/PEI-FA upconversion fluorescent nanoprobes had low cytotoxicity and were suitable for the application in HeLa cells targeted fluorescent imaging.