文献类型：期刊文献

英文题名：ROCK inhibitor attenuates carbon blacks-induced pulmonary fibrosis in mice via Rho/ROCK/NF-kappa B pathway

作者：Yan, Junyan[1];Tang, Yaxin[1];Zhong, Xin[1];Huang, Huarong[2];Wei, Haonan[1];Jin, Yulei[1];He, Yanjiang[1];Cao, Jinqiao[1];Jin, Lifang[1,3];Hu, Baowei[1]

机构：[1]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou, Peoples R China;[3]Zhejiang Sci Tech Univ, Shaoxing Acad Biomed, Hangzhou, Zhejiang, Peoples R China

年份：2021

卷号：36

期号：7

起止页码：1476

外文期刊名：ENVIRONMENTAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000635431300001)、、EI(收录号：20211510214380)、Scopus(收录号：2-s2.0-85103933874)、WOS

基金：Natural Science Foundation of Zhejiang Province, Grant/Award Number: LQ20B070003; Shaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Grant/Award Number: SXAB202015; Zhejiang Province Science and Technology Project of China, Grant/Award Number: 2018C37105

语种：英文

外文关键词：carbon black; NF‐ kappa B; pulmonary fibrosis; RhoA; rho kinase; Y‐ 27632

外文摘要：Exposure to carbon blacks (CBs) has been associated with the progression of pulmonary fibrosis, whereas the mechanism is still not clear. We therefore aimed to investigate the effect of RhoA/ROCK pathway on pulmonary fibrosis caused by CBs exposure. Western blot analysis indicated that CBs could promote the activation of RhoA/ROCK pathway and phosphorylation of p65 and I kappa B alpha in mice lung. However, ROCK inhibitor Y-27632 could attenuate phosphorylation levels of p65 and I kappa B alpha and restore histopathological changes of the lung tissue. Then, we evaluated the effect of RhoA/ROCK pathway on pulmonary fibrosis by detecting the expression levels of alpha-SMA, vimentin, and Collagen type-I (Col-I), which could be partly inhibited by Y-27632. It was assumed that inhibition of ROCK could be a promising therapeutic candidate for CBs-induced pulmonary fibrosis, which possibly through the blockage of RhoA/ROCK/NF-kappa B pathway.