文献类型：期刊文献

英文题名：A Novel Metallothionein Gene from Mussel, Hyriopsis cumingii: Identification and Expression under Lanthanum Exposure

作者：Yang, Shoubao[1];Wei, Min[1,2];Yang, Xiaoxue[3];Wang, Heng[1,2];He, Liping[1];Li, Chen[1]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China;[2]Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China;[3]Anhui Normal Univ, Coll Life Sci, Wuhu 241000, Peoples R China

年份：2014

卷号：45

期号：4

起止页码：454

外文期刊名：JOURNAL OF THE WORLD AQUACULTURE SOCIETY

收录：SCI-EXPANDED(收录号：WOS:000345346600011)、、WOS

基金：We thank the Natural Science Foundation of Zhejiang Province (LY12C19010) and the Major Science and the Technology Projects in Aquatic Breeding of Zhejiang Province (2012C12907-5 and 2012C12907-9) for financial support.

语种：英文

外文摘要：Metallothionein (MT) is a superfamily of cysteine-rich, metal-binding, low-molecular-weight proteins that participate in the storage, transportation, and metabolism of the trace metal elements. In this study, a novel MT gene from Hyriopsis cumingii was cloned (designated HcMT1); it shared 59.2% identity with previously published MT sequence of H. cumingii and 56.1-97.3% identity with other MT genes from mollusks. The expression level of HcMT1 in various tissues was determined by real-time polymerase chain reaction. Relatively higher expression levels were observed in digestive gonad, mantle, and gill, and the expression level in hemocytes was significantly upregulated by lanthanum (La3+) exposure. The nucleotide sequence of HcMT1 amplified from genomic DNA is identical to the cDNA template group under La3+ exposure, which indicated that HcMT1 is an intronless gene. These results indicate that HcMT1 may play an important role in La3+ metabolism.