文献类型：期刊文献

英文题名：Bergapten prevents lipopolysaccharide mediated osteoclast formation, bone resorption and osteoclast survival

作者：Zheng, Mingxia[1];Ge, Yongliang[1];Li, Haiyu[1];Yan, Ming[2];Zhou, Jin[1];Zhang, Yun[1]

机构：[1]Shaoxing Univ, Coll Med, Shaoxing 312000, Peoples R China;[2]Hang Zhou Dianzi Univ, Ctr Lab Biomanufacture & Tissue Engn, Hangzhou 310018, Zhejiang, Peoples R China

年份：2014

卷号：38

期号：3

起止页码：627

外文期刊名：INTERNATIONAL ORTHOPAEDICS

收录：SCI-EXPANDED(收录号：WOS:000334207400024)、、Scopus(收录号：2-s2.0-84898867904)、WOS

基金：This work was supported by Applied Research Program of Public Good in Zhejiang Province (No. 2011C33046), Science and Technology Project of Shaoxing City (No. 2011A33004), Zhejiang Provincial Natural Science Foundation of China (No. LQ12H18001 and No. Y2111046), Undergraduate Scientific and Technological Innovation Project in Zhejiang Province (No. 03160104) and Undergraduate Scientific and Technological Innovation Project in Shaoxing City (No. 2012R426024).

语种：英文

外文关键词：Bergapten; LPS; Osteoclasts; Bone resorption; Apoptosis

外文摘要：Purpose This study was designed to investigate the potential effect of bergapten on lipopolysaccharide (LPS)-mediated osteoclast formation, bone resorption and osteoclast survival in vitro. Methods After osteoclast precursor RAW264.7 cells were treated with bergapten (5, 20, 40 mu mol/L) for 72 hours in the presence of LPS (100 ng/ml), osteoclastogenesis was identified by tartrate-resistant acid phosphatase (TRAP) staining, and the number of TRAP-positive multinucleated cells [TRAP(+)MNCs] per well were counted. To investigate the effect of bergapten on osteoclastic bone resorption, RAW264.7 cells were treated with bergapten for six days in the presence of LPS, and the area of bone resorption was analyzed with Image Pro-Plus. Next, we examined apoptosis of RAW264.7 cells after bergapten incubation for 48 hours by flow cytometer using annexin V/propidium iodide (PI) double labeling. Finally, osteoclast survival was observed by Hoechst 33342 labeling and Western blotting after bergapten treatment for 24 hours. Results Data showed that bergapten (5-40 mu mol/L) dose-dependently inhibited LPS-induced osteoclast formation and bone resorption. Treatment with bergapten triggered apoptotic death of osteoclast precursor RAW264.7 cells in a dose-dependent manner. Furthermore, bergapten significantly reduced the survival of mature osteoclast, as demonstrated by emergence of apoptotic nuclei and activation of apoptotic protein caspase 3/9. Conclusions These findings suggest that bergapten effectively prevents LPS-induced osteoclastogenesis, bone resorption and survival via apoptotic response of osteoclasts and their precursors. The study identifies bergapten as an inhibitor of osteoclast formation and bone resorption and provides evidence that bergapten might be beneficial as an alternative for prevention and treatment of inflammatory bone loss.