文献类型：期刊文献

英文题名：Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice

作者：Hu, Chun Xia[1,2];Xu, Zi Rong[1];Li, Wei Fen[1];Dong, Niu[1];Lu, Ping[3];Fu, Ling Lin[1]

机构：[1]Zhejiang Univ, Key Lab Mol Anim Nutr, Minist Educ, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China;[2]Shaoxing Univ, Dept Life Sci, Yuanpei Coll, Shaoxing 312000, Zhejiang, Peoples R China;[3]Zhejiang Univ, Inst Microbiol, Coll Life Sci, Hangzhou 310029, Zhejiang, Peoples R China

年份：2009

卷号：31

期号：7

起止页码：991

外文期刊名：BIOTECHNOLOGY LETTERS

收录：SCI-EXPANDED(收录号：WOS:000267056600011)、、EI(收录号：20092712161707)、Scopus(收录号：2-s2.0-67649215128)、WOS

基金：This study was supported by Zhejiang Provincial Science and Technology Foundation, China (No. 2006C12086).

语种：英文

外文关键词：Enterotoxigenic Escherichia coli; FaeG; Immune response; Lactococcus lactis; Oral vaccination

外文摘要：K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis [spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C-83549 challenge were evaluated in ICR mice. Mice vaccinated with L. lactis [spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis [spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice.