文献类型：期刊文献

中文题名：OsPIN1a Gene Participates in Regulating Negative Phototropism of Rice Roots

英文题名：OsPIN1a Gene Participates in Regulating Negative Phototropism of Rice Roots

作者：XU Hua-wei[1];MO Yi-wei[2];WANG Wei[3];WANG Hai[2];WANG Zhong[4]

机构：[1]College of Agriculture,Henan University of Science and Techenology;[2]College of Life Science,Shaoxing University;[3]College of Life Science,Anhui Agricultural University;[4]College of Bioscience and Biotechnology,Yangzhou University

年份：2014

期号：2

起止页码：83

中文期刊名：水稻科学：英文版

收录：CSTPCD、、ESCI(收录号：WOS:000213879700002)、Scopus、WOS

基金：supported by the grants from the National Natural Science Foundations of China(Grant Nos.31071353 and 31100197);the Anhui Provincial Natural Science Fund of Youth,China(Grant No.1308085QC50);the Fund of Provincial Excellent Young Talents in Universities and Colleges,China(Grant No.2012SQRL057)

语种：英文

中文关键词：转基因植物;负向光性;水稻根;农杆菌介导转化;RT-PCR;融合表达载体;绿色荧光蛋白;GenBank

外文关键词：rice; OsPINla; green fluorescent protein; transient expression; negative phototropism

中文摘要：The complete open reading frame of OsPIN1a was amplified through reverse transcriptase- polymerase chain reaction(RT-PCR) based on the sequence deposited in GenBank to explore the relationship between the auxin efflux protein OsPIN1a and the negative phototropism of rice roots. Sequencing results showed that the GC content of OsPIN1a was 65.49%. The fusion expression vector pCAMBIA-1301-OsPIN1a::GFP containing the OsPIN1a gene and a coding green fluorescent protein(gfp) gene was constructed. The fusion vector was transferred into onion epidermal cells by Agrobacterium tumefaciens transformation. The transient expression of OsPIN1a-GFP was mainly located in the nucleus and cell membrane. Moreover, the transgenic plants were obtained by Agrobacterium-mediated genetic transformation. Molecular detection performed by using PCR and β-glucuronidase staining showed that the target construct was integrated into the genome of rice. The negative phototropic curvatures of the transgenic rice roots were higher than those of the wild type. Similarly, the expression levels of OsPIN1a in the transgenic plants were considerably higher than those in the wild-type plants. These results suggest that OsPIN1a is crucial in the negative phototropic curvature of rice roots.

外文摘要：The complete open reading frame of OsPINla was amplified through reverse transcriptase- polymerase chain reaction （RT-PCR） based on the sequence deposited in GenBank to explore the relationship between the auxin efflux protein OsPINla and the negative phototropism of rice roots. Sequencing results showed that the GC content of OsPINla was 65.49%. The fusion expression vector pCAMBIA-1301-OsP/N1a：：GFP containing the OsPINla gene and a coding green fluorescent protein （gfp） gene was constructed. The fusion vector was transferred into onion epidermal cells by Agrobacterium tumefaciens transformation. The transient expression of OsPINla-GFP was mainly located in the nucleus and cell membrane. Moreover, the transgenic plants were obtained by Agrobacterium-mediated genetic transformation. Molecular detection performed by using PCR and β-glucuronidase staining showed that the target construct was integrated into the genome of rice. The negative phototropic curvatures of the transgenic rice roots were higher than those of the wild type. Similarly, the expression levels of OsPINla in the transgenic plants were considerably higher than those in the wild-type plants. These results suggest that OsPINla is crucial in the negative phototropic curvature of rice roots.