文献类型：期刊文献

英文题名：Novel enzyme/exfoliated bentonite nanohybrids as highly efficient and recyclable biocatalysts in hydrolytic reaction

作者：Cao, Jie[1];Li, Yimin[1];Tu, Ni[1];Lv, Ying[1];Chen, Qinqin[2];Dong, Huaping[1]

机构：[1]Shaoxing Univ, Coll Chem & Chem Engn, 508 Huancheng West Rd, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Coll Med, 508 Huancheng West Rd, Shaoxing 312000, Zhejiang, Peoples R China

年份：2016

卷号：132

起止页码：41

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：SCI-EXPANDED(收录号：WOS:000382409300006)、、EI(收录号：20162902601889)、Scopus(收录号：2-s2.0-84978033964)、WOS

基金：This work was supported by the National Natural Science Foundation of China (21177088), the Natural Science Foundation of Zhejiang Province, China (LY16B070004), and the Research Project for Public Welfare of Zhejiang Province, China (2015C33229).

语种：英文

外文关键词：Bentonite; Enzyme immobilization; Exfoliation; Nanohybrid; Recyclability

外文摘要：Bentonite exfoliation liberated the interlayer surface with large area for immobilization of enzyme at high enzyme loading. Upon the electrostatic attraction between positively charged bovine pancreatic lipase (BPL), Yarrawia lipolytica lipase (YLL), trypsin and negatively charged surface of unilaminar bentonite, three nanohybrids with a stably card-like structure named as BPL-B, YLL-B and TB were facilely produced. These nanohybrids showed higher activities in hydrolysis as 115.2% (BPL-B), 154.8% (YLL-B) and 138.2% (TB) of that of the correspondingly free enzyme. Furthermore, BPL-B, YLL-B and TB retained 80%, 90% and 82% of each original activity after repeated use for 10, 12 and 8 times, respectively. The recyclable and active nanohybrids are expected to be widely applied as industrial catalysts in the future. (C) 2016 Elsevier B.V. All rights reserved.