文献类型：期刊文献

英文题名：Auxin efflux carriers, MiPINs, are involved in adventitious root formation of mango cotyledon segments

作者：Li, Yun-He[1];Mo, Yi-Wei[2];Wang, Song-Biao[1];Zhang, Zhi[1]

机构：[1]Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang 524091, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China

年份：2020

卷号：150

起止页码：15

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000527876200002)、、Scopus(收录号：2-s2.0-85079672795)、WOS

基金：We thank Prof. Pingfang Yang and Dr. Ming Li (Wuhan Botanical Garden, Chinese Academy of Sciences) for their help with Western blot analysis. This work was financially supported by the National Key Research and Development Program of China (2018YFD1000504) and the National Natural Science Foundation of China (31372053).

语种：英文

外文关键词：Adventitious root; Mangifera indica; MiPINs; Polar auxin transport

外文摘要：Adventitious roots form only at the proximal cut surface (PCS) but not at the distal cut surface (DCS) of mango cotyledon segments. In this study, mango embryos treated with indole-3-butyric acid (IBA) showed significantly increased adventitious root formation, while those treated with 2, 3, 5-triiodobenzoic acid (TIBA) demonstrated complete inhibition of adventitious rooting. Mango embryos treated with auxin influx inhibitors demonstrated lower inhibition of adventitious roots than those treated with TIBA. The endogenous indol-3-acetic acid (IAA) content on the PCS and DCS was similar at 0 h, then increased on both surfaces after 6 h, and IAA content on the PCS were always higher than those on the DCS. We cloned three genes encoding auxin efflux carriers (i.e., MiPIN2-4) and examined their temporal and spatial expression patterns under different treatments. Relative expression of all MiPINs studied was very low at 0 h but significantly increased on both PCS and DCS from 1 d to 10 d, to varying degrees. We overexpressed MiPIN1-4 in Arabidopsis plants and found a significant increase in adventitious root quantity in MiPIN1 and MiPIN3 transgenic lines. Immunofluorescence results showed that MiPIN1 and MiPIN3 are primarily localized in the vascular tissues and the cells adjacent to abaxial surface. In conclusion, we propose that in mango cotyledon segments, wounding stimulates IAA biosynthesis, the transcription levels of PIN genes were significantly increased in different magnitudes on the PCS and DCS, resulting in polar IAA transport from the DCS to PCS via the vascular tissues, thereby triggering adventitious root formation.