文献类型：期刊文献

英文题名：Enhanced production of diosgenin from Dioscorea zingiberensis in mixed culture solid state fermentation with Trichoderma reesei and Aspergillus fumigatus

作者：Cheng, Yutong[1];Dong, Chun[1];Huang, Chengcai[1];Zhu, Yuling[1,2]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing, Peoples R China;[2]Shaoxing Univ, Sch Chem & Chem Engn, Key Lab Clean Dyeing & Finishing Technol Zhejiang, Shaoxing, Peoples R China

年份：2015

卷号：29

期号：4

起止页码：773

外文期刊名：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT

收录：SCI-EXPANDED(收录号：WOS:000356960300023)、、WOS

基金：National Natural Science Foundation of China [grant number 21206093].

语种：英文

外文关键词：solid state fermentation; mixed culture; diosgenin; Trichoderma reesei; Aspergillus fumigatus; Dioscorea zingiberensis

外文摘要：Diosgenin is a valuable precursor for the synthesis of steroid compounds and is widely used in the pharmaceutical industry. The production of diosgenin from plant tubers by using microbial transformation is a cheap and environmentally-friendly method. However, the low efficiency of the existing fermentation protocols prevents the large-scale production required to meet the industrial demand. In this work, a solid state fermentation of Dioscorea zingiberensis was performed by using a mixed culture of Trichoderma reesei and Aspergillus fumigatus to increase the bioconversion efficiency. We optimized several parameters of the fermentation process of T. reesei and A. fumigatus monocultures using a response surface methodology. Next, we tested fermentation strategies and different ratios of T. reesei:A. fumigatus (T:A) inoculum. A maximum yield (95.82%) resulted from using a T:A ratio of 1:2 (7.5 x 10(6)T. reesei spores/g pretreated DZW) and a fermentation strategy, in which T. reesei was added at the start of the fermentation and A. fumigatus was added after 24 hours.