文献类型：期刊文献

中文题名：大鼠睾丸Cx43蛋白表达的加龄变化

英文题名：Aging changes in Cx43 expression in rat testis

作者：陈珊珊[1];张金萍[1];郑刚[1];胡圣佳[1];戴圣倩[1];陈徐[1]

机构：[1]绍兴文理学院医学院临床医学系

年份：2016

卷号：39

期号：1

起止页码：19

中文期刊名：解剖学杂志

外文期刊名：Chinese Journal of Anatomy

收录：CSTPCD、、北大核心2014、北大核心、CSCD_E2015_2016、CSCD

基金：2013年浙江省大学生科技创新活动计划暨新苗人才计划(2013R426024)

语种：中文

中文关键词：发育;睾丸;精子;Cx43蛋白;大鼠

外文关键词：development; testis; sperm; Cx43 protein; rat

中文摘要：目的：研究Cx43蛋白在大鼠出生后不同周龄睾丸中表达和定位，探讨Cx43蛋白与大鼠睾丸发育和精子发生的相关性。方法：应用免疫组织化学研究Cx43蛋白在1、3、5、7、9、11、13周龄SD雄性大鼠睾丸中的表达，利用血细胞计数板检测5～13周龄精子的数量。结果：Cx43蛋白于第1周开始在支持细胞、精原细胞中表达，第3周在精母细胞中表达，第5周开始在间质细胞、精母细胞、精子细胞中表达，随周龄增加而表达增加；第5周开始出现精子，随周龄增加而增加，Cx43蛋白表达与精子数量呈正相关。结论：Cx43蛋白在大鼠睾丸发育和精子发生的各个阶段均有表达，提示其参与睾丸发育和精子发生的调节。

外文摘要：Objective： To investigate the expression and localization of eonnexin 43（Cx43） in rat testis in different weeks, and the relationship between Cx43 and testicular development and spermatogenesis in rats. Methods ： The expression of Cx43 of male SD rat testis in 1,3,5,7,9,11,13 weeks were studied by immunohistochemistry. The number of sperms in 5 to 13 weeks＇ rats were determined with blood cell counting plate. Results ： Cx43 began to express in Sertoli cells and spermatogonias in the first week, in spermatocytes in the third week, and in mesenchymal cells, spermatocytes and sperm cells in the fifth week, which increased with age. In the fifth week, sperms began to appear, which increased with age. And Cx43 protein was positively associated with the number of sperms. Conclusion： The expression of Cx43 protein appeares in different stages of testicular development and spermatogenesis in rats, which suggests that it be involved in the regulation of testicular development and spermatogenesis.