文献类型：期刊文献

英文题名：Positive Regulation of the Transcription of AchnKCS by a bZIP Transcription Factor in Response to ABA-Stimulated Suberization of Kiwifruit

作者：Han, Xueyuan[1,2];Mao, Linchun[2,3];Lu, Wenjing[4];Wei, Xiaopeng[2];Ying, Tiejin[2];Luo, Zisheng[2]

机构：[1]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Zhejiang Univ, Key Lab Agroprod Postharvest Handling, Zhejiang Key Lab AgroFood Proc, Coll Biosyst Engn & Food Sci,Minist Agr & Rural A, Hangzhou 310058, Zhejiang, Peoples R China;[3]Zhejiang Univ, Ningbo Res Inst, Ningbo 315100, Zhejiang, Peoples R China;[4]Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China

年份：2019

卷号：67

期号：26

起止页码：7390

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000474669600019)、、WOS

基金：This research was supported by the National Natural Science Foundation of China (31772365) and the National Basic Research Program (973 program) of China (2013CB127101).

语种：英文

外文关键词：beta-ketoacyl-coenzyme A synthases (KCSs); bZIP; abscisic acid (ABA); kiwifruit; suberization

外文摘要：Wound-induced suberization is an essentially protective healing process for wounded fruit to reduce water loss and microbial infection. It has been demonstrated that abscisic acid (ABA) could promote wound suberization, but the molecular mechanism of ABA regulation remains little known. In this study, the transcript level of Achn030011 (designated as AchnKCS), coding a beta-ketoacyl-coenzyme A synthase (KCS) involved in suberin biosynthesis, was found to be significantly upregulated by ABA in wounded kiwifruit. A bZIP transcription factor (Achn270881), a possible downstream transcription factor in the ABA signaling pathway, was screened and designated as AchnbZIP12 according to its homology with related Arabidopsis transcription factors. A yeast one-hybrid assay demonstrated that AchnbZIP12 could interact with the AchnKCS promoter. Furthermore, significant trans-activation of AchnbZIP12 on AchnKCS was verified. The transcript level of AchnbZIP12 was also upregulated upon treatment with ABA. These results imply that AchnbZIP12 acts as a positive regulator in ABA-mediated AchnKCS transcription during wound suberization of kiwifruit.