文献类型：期刊文献

英文题名：Ultrastructural and immunocytochemical analysis of circulating hemocytes from Cherax quadricarinatus (von Martens, 1868)

作者：Zeng Wentao[1];Luo Wen[1];Zhao Yunlong[1,2];Wang Danli[1,2];Mi Zhongxiang[1];Shi Getao[1]

机构：[1]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Peoples R China;[2]East China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

年份：2017

卷号：51

期号：1

起止页码：129

外文期刊名：INDIAN JOURNAL OF ANIMAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000394071400024)、、WOS

基金：The project was supported by Agro-scientific Research in the Public Interest (No. 201203081), Zhejiang Provincial Natural Science Foundation of China under Grant (No. LY16C190002) and Science Technology Bureau of Shaoxing (No. 2012B70030). We thank the Lucidus Consultancy for language revision of this paper.

语种：英文

外文关键词：Circulating hemolytic; CD34+cells; Density gradient centrifugation

外文摘要：Transmission electron microscopy (TEM) and immunocytochemistry were used to assay the circulating hemocytes characteristics in Cherax quadricarinatus (von Martens, 1868). Three haemocyte types could be identified in circulating hemocytes by TEM, i.e. hyaline cells (HC), semigranular cells (SGC), or granular cells (GC). The circulating hemocytes from C. quadricarinatus could be separated into layers by density gradient centrifugation. The CD34+ cells which are believed to be stem cell-like cells has been discovered in the first layer via immunocytochemical assay, which has not been reported in previous studies. These CD34+ cells may be immature cells derived from a single undifferentiated precursor cell line in specialized hematopoietic tissue (HPT).