文献类型：期刊文献

英文题名：Thermal and environmental stability of Siniperca chuatsi Rhabdovirus

作者：Xu, Zhendong[1];Huang, Zhiyang[1];Zhong, Junyao[1];Zhu, Yinzhi[1];Liu, Xiaoyu[1];Wei, Yongwei[1,2]

机构：[1]Shaoxing Univ, Sch Med, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Sch Med, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China

年份：2023

卷号：568

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000934901200001)、、WOS

基金：This study was supported by the National Natural Science Founda- tion of China (31402218) , the Natural Science Foundation of Zhejiang Province (LY14C180001) , and the National Innovation and Entrepre- neurship Training Program for Undergraduates (202110349032) .

语种：英文

外文关键词：SCRV; Thermal stability; Liquids; Solid surface; UV irradiation

外文摘要：Siniperca chuatsi Rhabdovirus (SCRV) is an important pathogen that can cause huge economic losses and serious threats to aquaculture in mandarin fish and other economic fish. This study evaluated the stability of SCRV in liquids and on dry solid surfaces and explored the mechanism of SCRV inactivation by thermal treatment and UV irradiation. SCRV in the DMEM medium environment was very stable at 4 degrees C with a decrease of only 1 log10 for 6 months. The virus titer was still as high as 3 x 103 PFU/mL after 1 year. It survived for 14 days at 37 degrees C, and survived for 60 days and 150 days at 25 degrees C and 15 degrees C, respectively. The stability of SCRV in ddH2O was close to that in DMEM, and the stability in pond water was weaker than that in ddH2O and DMEM. On the surfaces of plastic (polystyrene), glass, and stainless steel, SCRV survived for 6 days at 25 degrees C and could survive for up to 1 month at 4 degrees C. The commonly used procedure of heat inactivation at 55 degrees C for 30 min is not suitable for SCRV. The complete thermal inactivation for SCRV was 60 degrees C for 30 min or 65 degrees C for 5 min. UV irradiation (wavelength 254 nm, intensity 400 mW/m2, irradiation distance 75 cm) for 120 s can effectively inactivate SCRV. RT-PCR analysis of the G gene indicated that the lost infectivity of SCRV was in part due to the disruption of genomic RNA. These findings are of great value for the prevention and control of SCRV outbreaks, and also have important reference value for disease control in other fisheries.