文献类型：期刊文献

中文题名：神经纤维丝蛋白、突触素在人胚胎脊髓发育阶段的表达

英文题名：Expression of neurofilament protein and synaptophysin in the developing spinal cord of human embryo

作者：张泳[1];刘学红[1]

机构：[1]绍兴文理学院医学院

年份：2014

卷号：45

期号：4

起止页码：545

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014

基金：浙江省公益性技术应用研究计划资助项目(2012C33066);绍兴市公益性技术应用研究计划资助项目(2013D10035)

语种：中文

中文关键词：胚胎;脊髓;神经纤维丝蛋白;突触素;免疫组织化学;人

外文关键词：Embryo;Spinal cord;Neurofilament protein;Synaptophysin;Immunohistochemistry;Human

中文摘要：目的：探讨神经纤维丝蛋白（ NF）、突触素（ SYN）在人胚胎脊髓发育阶段的分布规律及其表达意义。方法应用免疫组织化学法检测第2、3、4个月龄段，共16例人胚胎脊髓中NF、SYN的表达、分布状况。结果第2个月胚龄时，NF仅在人胚脊髓的前正中裂和后正中隔两侧的边缘层呈弱阳性表达。第3～4月龄时，NF在脊髓的前正中裂、后正中隔及皮质区域均呈阳性或强阳性表达，在室管膜层外侧的部分灰质内可见NF表达阳性的神经纤维。第2～4个月龄段，人胚胎脊髓内均可见SYN阳性表达，随着胎龄的增大，脊髓内SYN阳性表达强度值呈先升高再降低，阳性数量值呈逐月升高。结论 NF和SYN参与调控人胚胎脊髓组织的生长发育。

外文摘要：Objective To investigate the neurofilament protein （NF） and synaptophysin（SYN） distribution in the developing stage of human embryonic spinal cord and their clinical significance . Methods Totally 16 of human embryos, aged from 2 to 4 months were used in this study .Immunohistochemical staining was uesd to detect the expression and distribution of NF and SYN in the spinal cord .Results At the second month stage , NF expression was weakly positive in only the marginal layer on both sides of the spinal cord .At 3-4 months, NF expression was positive or strong positive in the spinal cord center crack before and after the middle of partition and cortex .In the ependymal layer of the lateral part of the expression of NF positive nerve fibers were seen in the gray matter .From 2 to 4 month-age, all visible SYN positive expressions distributed in the spinal cord .With the increase of gestational age , SYN positive expression in the spinal cord staining intensity value was increased first , and then decreased .The number of positive values increased month by month . Conclusion NF and SYN both take part in the regulation of human embryonic spinal cord tissue growth .