文献类型：期刊文献

中文题名：姜黄素类似物L6H4对2型糖尿病大鼠膈肌的保护作用

英文题名：Protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats

作者：项兰婷[1];顾倩如[1];张仕锵[1];董细丹[1];应丽丽[1];陈三妹[2];陈国荣[1]

机构：[1]温州医科大学附属第一医院病理科;[2]绍兴文理学院医学院

年份：2017

卷号：33

期号：10

起止页码：1806

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD、、北大核心2014、CSCD2017_2018、北大核心、CSCD

基金：浙江省公益性技术应用研究计划(No.2011C23123)

语种：中文

中文关键词：糖尿病;膈肌;姜黄素类似物;脂联素;脂联素受体1

外文关键词：Diabetes mellitus; Diaphragm; Curcumin analogues; Adiponectin; Adiponectin receptor 1;

中文摘要：目的:探讨姜黄素类似物L6H4对2型糖尿病大鼠膈肌的保护作用及机制。方法:40只SPF级雄性SD大鼠,随机均分成5组:正常对照(NC)组、高脂(HF)组、高脂治疗(FT)组、糖尿病(DM)组和糖尿病治疗(DT)组。后4组采用高脂饲料喂养,4周后DM组及DT组腹腔注射链脲佐菌素诱导2型糖尿病模型,造模成功后FT组和DT组用L6H4灌胃治疗8周。生化法检测空腹血糖及血脂水平;放射免疫法检测空腹血清胰岛素(FINS)水平并计算胰岛素抵抗指数(HOMA-IR);ELISA法检测血清脂联素(APN)水平;光镜和电镜观察大鼠膈肌形态改变;酶组织化学染色观察膈肌内脂质沉积及琥珀酸脱氢酶(SDH)和还原型辅酶Ⅰ四氮唑还原酶(NADH-TR)活性;硫代巴比妥酸法和羟胺法分别检测膈肌丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;免疫组化和Western blot检测膈肌脂联素受体1(AdipoR1)蛋白的表达。结果:HF组和DM组大鼠的血糖、血脂、FINS和HOMA-IR较NC组均有升高(P<0.01),L6H4治疗后均下降(P<0.01);HF组与DM组大鼠血清APN水平较NC组下降,L6H4治疗后升高(P<0.01)。HF组及DM组大鼠膈肌纤维萎缩,肌纤维内脂肪颗粒堆积,线粒体轻微肿胀;DM组大鼠膈肌纤维化,L6H4治疗后,大鼠膈肌的形态学损害减轻。HF组和DM组大鼠膈肌MDA含量及SDH和NADH-TR活性较NC组升高,L6H4治疗后下降(P<0.05);HF组和DM组大鼠膈肌SOD活性和AdipoR1蛋白表达较NC组下降,L6H4治疗后升高(P<0.05)。结论:姜黄素类似物L6H4对2型糖尿病大鼠的膈肌具有保护作用;膈肌AdipoR1蛋白表达增强、血清APN浓度增加及抗脂质过氧化可能参与其中。

外文摘要：AIM：To investigate the protective effect of curcumin analogue L 6H4 on diaphragm of type 2 dia-betic rats.METHODS： SPF male Sprague-Dawley rats （ n=40） were randomly divided into 5 groups： normal control （NC） group, high fat （HF） group, high fat＋L6H4 treatment （FT） group, diabetes mellitus （DM） group and DM ＋L6H4 treatment （DT） group.The rats in the later 4 groups were fed with high-fat diet.After 4 weeks of high-fat diet fee-ding, the rats in DM and DT groups were intraperitoneally injected with streptozotocin to induce type 2 diabetes melliutus. The rats in FT and DT groups were given L6H4 by gavage for 8 weeks.Blood glucose and blood lipid levels were detected biochemically .Fasting serum insulin （ FINS ） level was measured by radioimmunoassay and insulin resistance index （HOMA-IR） was calculated.Serum adiponectin （APN） level was measured by ELISA.The morphological changes of the diaphragm were observed under light and transmission electron microscopes .Lipid deposition and the activity of succinate dehydrogenase （SDH） and NADH-tetrazolium reductase （NADH-TR） were observed by enzyme histochemical staining . The content of malondialdehyde （ MDA） and the activity of superoxide dismutase （ SOD） in the diaphragm were measured by thiobarbituric acid method and hydroxylamine method , respectively .The protein expression of adiponectin receptor 1 （ AdipoR1） in the diaphragm was determined by immunohistochemistry and Western blot .RESULTS：The levels of blood lipids , blood glucose , FINS and HOMA-IR in HF and DM groups were higher than those in NC group , but decreased after L6H4 treatment.The serum APN level in HF and DM groups was lower than that in NC group , but increased after treat-ment with L6H4.The muscle fibers of the diaphragm were shrunk , fat particles accumulated in the muscle fibers , and the mitochondria were slightly swollen in HF and DM groups .The diaphragmatic fibrosis was obvious in DM group .These le-sions were relieved after L6H4 treatment.Compared with NC group, the level of MDA and the activity of SDH and NADH-TR in the diaphragm were increased in HF and DM groups , but decreased after treatment with L 6H4.The activity of SOD and the expression of AdipoR1 in the diaphragm were lower than those in NC group , but increased after L6H4 treatment. CONCLUSION： The curcumin analogue L6H4 exerts a protective effect on diaphragm in type 2 diabetic rats.The strengthened protein expression of AdipoR 1, the increased serum level of APN , and anti-lipid peroxidation may be involved in the process .