文献类型：期刊文献

中文题名：HPLC法测定黑龙江省不同地区刺五加紫丁香苷的含量

英文题名：Syringin content of Acanthopanax senticosus samples in Heilongjiang different zones measuring by HPLC method

作者：张艳华[1];孙立夫[1];施维林[1];王奇超[2]

机构：[1]绍兴文理学院;[2]东北林业大学森林植物生态学教育部重点实验室

年份：2006

卷号：26

期号：1

起止页码：123

中文期刊名：植物研究

外文期刊名：Bulletin of Botanical Research

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：哈尔滨市青年基金资助

语种：中文

中文关键词：刺五加;紫丁香苷;HPLC

外文关键词：Acanthopanax senticosus ; syringin ; HLPC

中文摘要：刺五加(Acanthopanax senticosus(Rupr.EtMaxim.)Harms)是五加科植物,是全球畅销的植物药之一,含有大量生物活性物质,其中所含有的刺五加苷类具有抗疲劳、抗衰老、抑制肿瘤生长等作用,紫丁香苷则是其中的主要活性成分之一。用HPLC法测定黑龙江省不同地区刺五加中紫丁香苷的含量,确定了刺五加紫丁香苷用超声波提取1.5 h、60%甲醇作提取溶剂、提取温度为55℃的最佳提取条件组合。HPLC条件则是以检测波长为266 nm、流动相为甲醇/水(V/V)=28/72、流速为1 mL.m in-1、柱温为25℃。对黑龙江省不同地区类似生境条件下,同龄野生刺五加中紫丁香苷的含量进行比较,在所调查的7个地区中,以五营丰林国家自然保护区的生长在红松原始林下的刺五加紫丁香苷含量最高,五大连池雪洞风景区和牡丹江镜泊湖地区的刺五加紫丁香苷的含量最低。该研究结果对刺五加的采集和种植生境的选择具有积极的指导意义。

外文摘要：Acanthopanax senticosus （ Rupr. et Maxim） Harms belongs to Araliaceae, which is a popular plant medicine with lots of bioactive substance around the world. Eleutheroside has many functions, such as resisting weariness and senescence, restraining tumor growth etc. Syringin is a main component of eleutheroside. Ultrasonic extraction method with 1.5 hour, sixty percent carbinol liquor as extractant and 55℃ are the best combination of extraction method. HPLC suitable condition was 266 nm as measuring wavelenghth, carbinol and water（28：72） used as mobile phase, 1 mL ·min^-1 as velocity of flow, column temperature with 25℃. Syringin content in root and stem were compared and analyzed in similar habitats in Heilongjiang different zones. The results showed that in the seven investigation zones, syringin content of this species was highest in Wuying National Nature Reserve, which grew under Pinus koraiensis original forest, but lowest in Snow Hollow Beauty Spot of Wudalianchi and Miror Lake area of Mudan river. This result would be benefit on gathering many-prickle acanthopanax and selecting the cropping habitats.