文献类型：期刊文献

英文题名：Kaempferol alleviates acute alcoholic liver injury in mice by regulating intestinal tight junction proteins and butyrate receptors and transporters

作者：Chen, Jing[1];Xuan, Yan-han[1];Luo, Ming-xiao[1];Ni, Xiang-gui[1];Ling, Li-qian[1];Hu, Shi-jia[1];Chen, Jing-qiao[1];Xu, Jia-yi[1];Jiang, Li-ya[1];Si, Wen-zhang[2];Xu, Lin[3];Gao, Hui[4];Liu, Zheng[1,4];Li, Haiyu[1]

机构：[1]Shaoxing Univ, Dept Forens Toxicol, Forens Ctr, 900 Chengnan Rd, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Dept Gen Surg, Shaoxing Municipal Hosp, Shaoxing 312000, Zhejiang, Peoples R China;[3]Shaoxing Univ, Clin Lab, Shaoxing Municipal Hosp, Shaoxing 312000, Zhejiang, Peoples R China;[4]Shaoxing Univ, Dept Pharmacolgoy, Med Coll, Shaoxing 312000, Zhejiang, Peoples R China

年份：2020

卷号：429

起止页码：152338

外文期刊名：TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000508742400009)、、Scopus(收录号：2-s2.0-85077107679)、WOS

基金：This work was funded by the National Natural Science Foundation of China (Nos. 81560059 and 81760058), the Science & Technology Planning Project of Shaoxing City, China (Nos. 2017B70050), and the Scientific Research Fund of Shaoxing University, China (Nos. 20125025). We also thank Accdon (www.accdon.com) for linguistic assistance during the preparation of this manuscript and Yuying Yao for her excellent generation of HE & IMH staining slices.

语种：英文

外文关键词：Kaempferol; Acute alcoholic liver injury; Intestine barrier; Tight junction proteins; Butyrate receptor and transporter

外文摘要：An impaired gut-liver axis is a potential factor that contributes to alcoholic liver disease. Specifically, ethanol decreases intestinal integrity and causes gut dysbiosis. Butyrate, a fermentation byproduct of gut microbiota, is negatively altered following acute ethanol exposure. This study aimed to determine whether kaempferol could protect against alcoholic liver injury (AALI) in mice by regulating tight junction (TJ) proteins and butyrate receptors and transporters in intestines. Male Institute of Cancer Research (ICR) mice were randomly divided into five treatment groups: control, ethanol administered (5 g/kg), and the low-, medium- and high-dosage kaempferol (25, 50, 100 mg/kg) treatments. Intestinal expression was evaluated for the TJ proteins ZO-1 and occludin and the butyrate receptor GPR109A and butyrate transporter SLC58A proteins, in addition to plasma ALT and AST levels and pathomorphological changes in liver and intestinal tissues. The expression of the TJ proteins ZO-1 and occludin, butyrate receptors, and butyrate transporters in the ileum and proximal colon decreased in AALI mice, while plasma ALT and AST levels markedly increased. Kaempferol supplementation reversed these effects. These results suggest that kaempferol could serve as a prophylactic treatment against AALI in mice by increasing the expression of butyrate receptors, transporters, and TJ proteins in the intestinal mucosa.