文献类型：期刊文献

中文题名：中国与欧洲高卢蜜环菌的遗传多样性

英文题名：Genetic diversity of Armillaria gallica isolates from China and Europe revealed with ISSR analysis

作者：孙立夫[1];裴克全[2];张艳华[1];赵俊[3];杨国亭[4];秦国夫[3];宋玉双[3];宋瑞清[4]

机构：[1]绍兴文理学院生命科学学院;[2]中国科学院植物研究所植被与环境变化国家重点实验室;[3]国家林业局森林病虫害防治总站;[4]东北林业大学林学院

年份：2012

卷号：20

期号：2

起止页码：224

中文期刊名：生物多样性

外文期刊名：Biodiversity Science

收录：CSTPCD、、北大核心2011、CSCD2011_2012、北大核心、CSCD

基金：浙江省自然科学基金(Y3090409);哈尔滨市青年科学基金(2002AFQXJ006)

语种：中文

中文关键词：Armillaria;gallica;遗传多样性;遗传相似性;聚类分析;地理隔离

外文关键词：Armillaria gallica; genetic diversity; genetic similarity; clustering analysis; geographic isolation

中文摘要：高卢蜜环菌(Armillaria gallica)为北半球广布种,不同大陆间的菌株遗传相似性和多样性水平能反映出该种在洲际大陆尺度上的地理遗传变异关系。作者用ISSR(inter-simple sequence repeat)分子标记技术,对从中国和欧洲收集到的高卢蜜环菌79个菌株进行了遗传多样性分析。用6个ISSR引物扩增得到210个位点,其中多态性位点(频率<0.95)为202个,占96.2%,平均每个引物多态位点多达33.6个,表明ISSR标记在蜜环菌中存在较高的多态性。根据非加权类平均法(UPGMA)聚类分析,中国53个菌株中的49个在0.773的相似性水平上聚成了中国类群(China group);而欧洲26个菌株遗传分化较大,分别在0.775和0.763的相似性水平上聚成了欧洲类群A(Europe group A)和B(Europe group B);2个欧洲类群间的相似性水平仅为0.738,而欧洲类群A与中国类群间的相似性却达到了0.770;两个大陆均有少数菌株表现出较为明显的遗传分化,个别菌株的种内遗传相似性甚至低于蜜环菌种间的遗传相似性。结果表明,中欧两个大陆间的A.gallica菌株因地理隔离已经表现出明显的遗传分化,处于异域物种形成过程中;欧洲大陆的菌株遗传分化更为明显,可能是两个大陆A.gallica菌株的起源地。

外文摘要：Armillaria gallica is a cosmopolitan fungal species found in the northern hemisphere,and genetic diversity and similarity between different continental isolates of the species can reveal important large-scale biogeographical patterns.A total of 79 isolates of A.gallica were collected from Europe and China.We estimated the genetic diversity of these isolates using ISSR（inter-simple sequence repeat） markers.Six ISSR primers were employed to identify a total of 210 scorable fragments,of which 202（96.2%） were polymorphic loci（frequecy0.95）.The average number of polymorphic loci detected with each primer was 33.6.The results indicated that ISSR markers can reflect abundant polymorphism in Armillaria.According to UPGMA clustering analysis,49 out of 53 Chinese A.gallica isolates clustered into a China Group at the 0.773 similarity level.The genetic divergence of European isolates was significantly higher than predicted;these isolates were clustered into Europe Group A and Group B at the 0.775 and 0.763 similarity level,respectively.Thesimilarity coefficient between the two European groups was only 0.738,but it was 0.770 between China Group and Europe Group A.Some isolates from China and Europe had experienced obvious genetic divergence,and several isolates had lower intraspecific similarity coefficients than interspecific similarity coefficient found in other Armillaria biological species.Our results indicated that considerable genetic differentiation existed between European and Chinese isolates due to geographic isolation,and that these isolates were in the process of allopatric speciation.The differentiation among isolates of A.gallica from Europe was more evident,and Europe is possibly the place of origin for isolates of the species.