文献类型：期刊文献

中文题名：神经元特异性烯醇化酶和突触素在人胚胎食管组织发育阶段的表达

英文题名：Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos

作者：刘学红[1];张泳[1];刘传康[1]

机构：[1]绍兴文理学院医学院

年份：2015

卷号：0

期号：2

起止页码：184

中文期刊名：浙江大学学报：医学版

收录：MEDLINE(收录号：26038138)、CSTPCD、、北大核心2014、CSCD2015_2016、Scopus(收录号：2-s2.0-84938539709)、北大核心、CSCD、PubMed

基金：浙江省教育厅科研计划(Y201018535)

语种：中文

中文关键词：食管/病理学;食管/胚胎学;磷酸丙酮酸水合酶;突触蛋白类;免疫组织化学

外文关键词：Esophagus/patholoy;Esophagus/embryology;Phosphopyruvate hydratase;Synapsins;Immunohistochemistry

中文摘要：目的：探讨神经元特异性烯醇化酶（ NSE）和突触素（ SYN）蛋白在人胚胎食管组织不同发育阶段的分布特征。方法：经伦理委员会批准，收集受精龄为7～16周的人胚胎标本16例，应用免疫组织化学染色法检测胚胎食管组织内NSE和SYN的表达，定量分析其变化规律。结果：第2～4个月胎龄时，肌间神经丛和黏膜下神经丛内细胞呈NSE阳性表达，阳性表达的数量和强度第3个月胎龄甚于第2个月胎龄，第4个月胎龄甚于第3个月胎龄。仅在第3个月胎龄时，食管黏膜上皮层内部分细胞呈NSE阳性表达。第2～4个月胎龄时， SYN在食管各层组织内均呈广泛阳性或强阳性表达，尤其在肌间神经丛和黏膜下神经丛更为明显。结论：在人胚胎食管发育阶段， NSE和SYN均参与食管壁神经系统的发育过程。

外文摘要：Objective： To investigate the expression of neuron-specific enolase （NSE） and synaptophysin （SYN） proteins in different developmental stages of human embryonic esophagus . Methods： Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age （ n=16 ） .One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age .Results： In fetuses with 2, 3 and 4 months of gestation , the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38 ±8.37,＆amp;nbsp;25.00 ±11.54 and 38.00 ±15.09, respectively;the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38 ±14.93, 62.25 ±18.59 and 56.44 ±14.70, respectively .NSE-positive cells were detected in the esophageal epithelium only at the third month.In the fetuses at 2, 3 and 4 months of gestation , SYN in all layers of esophageal tissue were positively or strong positively expressed , especially in the myenteric plexus and submucosal plexus .The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69 ±9.34, 51 .84 ±6 .10 and 46 .41 ±6 .44 , respectively .Conclusion： SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development .