文献类型：期刊文献

英文题名：Genetic diversity and population structure of common bean (Phaseolus vulgaris) landraces from China revealed by a new set of EST-SSR markers

作者：Xu, Shengchun[1];Wang, Guofu[2];Mao, Weihua[3];Hu, Qizan[1];Liu, Na[1];Ye, Lingwei[1];Gong, Yaming[1]

机构：[1]Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China;[2]Shaoxing Univ, Yuanpei Coll, Dept Life Sci, Shaoxing 312000, Peoples R China;[3]Zhejiang Univ, Ctr Anal & Measurement, Hangzhou 310058, Zhejiang, Peoples R China

年份：2014

卷号：57

起止页码：250

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：SCI-EXPANDED(收录号：WOS:000346213700043)、、WOS

基金：The research was supported by the National Natural Science Foundation of China (31000676, 31372072), Zhejiang Provincial Natural Science Foundation of China (LY12C15004), and Zhejiang Provincial Important Science & Technology Specific Projects (2012C12903).

语种：英文

外文关键词：Expressed sequence tag (EST); Genetic diversity; Microsatellite; Phaseolus vulgaris; Population structure

外文摘要：A new set of EST-SSR markers were developed and employed to analyze the genetic diversity and population structure of Phaseolus vulgaris in China. A total of 2452 microsatellites were identified in 2144 unigenes assembled from P. vulgaris ESTs, indicating that merely 6.9% of the 30,952 unigene sequences contained SSRs. Seventeen of 153 randomly designed EST-SSR primer pairs successfully amplified polymorphic products in 31 landraces from six major production provinces of China, with the mean number of alleles per locus of 2.700 and polymorphism information content of 0.378. The observed and expected heterozygosity ranged from 0.100 to 0.954 and 0.081 to 0.558, respectively. Using these markers, both an unrooted neighbor-joining tree and principal coordinates analysis showed that almost all of the landraces were separated according with their regional distribution. Moreover, population structure analysis revealed that all genotypes formed into three distinct clusters (k = 3), suggesting that geographic and climatic factors could provide diverse degrees of selection pressure. Accordingly, germplasm collection and cross breeding among different regions are suggested to accelerate the process of diverse germplasm creation and broaden germplasm resources of Chinese common bean. (C) 2014 Elsevier Ltd. All rights reserved.