文献类型：期刊文献

中文题名：温度和pH值对南美白对虾(Penaeus vannmei)消化酶活性的影响

英文题名：EFFECTS OF TEMPERATURE AND pH ON ACTIVITY OF DIGESTIVE ENZYMES IN SHRIMP PENAEUS VANNMEI

作者：沈文英[1];胡洪国[2];潘雅娟[1]

机构：[1]绍兴文理学院生物科学系;[2]绍兴县水产技术推广站

年份：2004

卷号：35

期号：6

起止页码：543

中文期刊名：海洋与湖沼

外文期刊名：Oceanologia Et Limnologia Sinica

收录：CSTPCD、、北大核心2000、Scopus、CSCD2011_2012、北大核心、CSCD

基金：国家高新技术研究发展计划 (86 3)资助项目 ;2 0 0 1AA6 2 2 0 6 0号

语种：中文

中文关键词：南美白对虾;温度;pH;消化酶活性

外文关键词：Penaeus vannmei,Temperature,pH,Activity of digestive enzymes

中文摘要：采用双因子交互的方法研究了不同温度和pH值对南美白对虾淀粉酶、蛋白酶和脂肪酶活性的影响。结果表明 ,南美白对虾肝脏、肠道、胃的淀粉酶最适温度分别为 30℃、35℃、30— 35℃ ,最适pH均为 6 2。肝脏、肠道、胃的蛋白酶最适温度分别为 5 0— 65℃、5 5— 65℃、60℃ ,肝脏蛋白酶最适pH为 8 5— 9 0 ,肠道蛋白酶最适pH为 7 5— 8 5 ,胃蛋白酶最适pH为2 0和 5 5— 6 5。脂肪酶的活力很小 ,肝脏、肠道、胃的脂肪酶最适温度均为 37℃ ,最适pH均为 7 7。同时测得南美白对虾肠道、肝脏、胃组织内的pH分别为 6 7— 7 0、5 9— 6 1、5 1—5 3。在三种消化酶各自最适的温度、pH值下 ,比较其活力大小 ,在肝脏、肠道、胃均为蛋白酶>淀粉酶 >脂肪酶。

外文摘要：Using temperature/pH two-factor cross method,activily of three digestive enzymes in liver,intestine and stomach of shrimp Penaeus vannmei with wet weight of (6.58±0.78)g were examined to find out the best temperature/pH environment. The resuhs showed that when pH value ranges was between 5.2—8.7,the most favorable temperature for the amylase in liver,intestine and stomaeh were 30,35 and 30—35℃ respectively. When temperature range was between 15—40℃,the most favorable pH values were all 6.2. The results indicated also that the amylase was conducive in weak acidic condition. The best temperatures in liver,intestine and stomach for the protease were 50—65℃,55—65℃ and 60℃ respectively when the reacting pH values range was between 4.5—9.0 in liver,intestine protease and 1.5—6.5 in stomach protease,The best pH values for the protease of liver were 8.5—9.0,and of intestine were 7.5—8.5,and of stomach were 2.0 and 5.0—6.5 when the reacting temperature range was 25—65℃. These resuhs indicated that the most favorable temperatures in liver,intestine and stomach for protease were higher than growth temperature of P. vannmei Furthermore,the protease in liver and intestine were conducive to alkaline condition while the protease in stomach was conducive to acidic condition. The activny of lipase was weak and lower than that of protease and amylase. The oplimum temperature in liver,intestine and stomach for the lipase all were 37℃ when the reacting pH values range was between 5.7—8.2,and the optimum pH values all were 7.7 when the reacting temperature range was between 25—45℃. The result indicated that the lipase was conductive to weak alkalinity. The pH values in intestine,liver and stomach of P. vannmei measured in our experiment were 5.9—6.1 (6.08±0.04),6.7—7.0 (6.82±0.04) and 5.1—5.3 (5.22±0.04) respectively. This result indicated that the pH values in digestive organs of P. vannrnei were weak acidic. All the activity of three digestive enlzymes was in an order of from strong to weak:protease,amylase,lipase in liver,intestine and stomach at the most favorable reacting temperature and pH.