文献类型：期刊文献

英文题名：In vitro generation of myofibroblasts-like cells from liver epithelial progenitor cells of rhesus monkey (Macaca mulatta)

作者：Ji, Shaohui[1,3,4];Wang, Xihong[3,4,7];Shu, Jianhong[2];Sun, Aijing[5,6];Si, Wei[3,4];Guo, Xiangyu[3,4];Zhao, Bo[3,4,7];Ji, Weizhi[3,4];Jin, Lifang[1,3,4]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[2]Zhejiang Sci Tech Univ, Sch Life Sci, Hangzhou, Zhejiang, Peoples R China;[3]Chinese Acad Sci, Kunming Primate Res Ctr, Kunming, Yunnan, Peoples R China;[4]Chinese Acad Sci, Kunming Inst Zool, Kunming, Yunnan, Peoples R China;[5]Shaoxing Univ, Affiliated Hosp 1, Shaoxing 312000, Zhejiang, Peoples R China;[6]Shaoxing Univ, Shaoxing Peoples Hosp, Dept Pathol, Shaoxing 312000, Zhejiang, Peoples R China;[7]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

年份：2011

卷号：47

期号：5-6

起止页码：383

外文期刊名：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

收录：SCI-EXPANDED(收录号：WOS:000291256200008)、、Scopus(收录号：2-s2.0-80051667679)、WOS

基金：We wish to acknowledge Mr. Li Jian for assistance in confocal microscopic analysis. This work was supported by research grants from Zhejiang Provincial Natural Science Foundation of China Y2110911, National 863 High Technology Research and Development Program of China 2005AA219010, 2006AA02A101, and 2006AA02A116, Major State Basic Development Program 2006CB701505 and 2007CB947701, The Chinese Academy of Sciences KSCX1-YWR-47 and KSCX1-05-02, R&D Infrastructure and Facility Development Program of Yunnan Province 2006PT08-2, Social Science and Technology Development Program of Yunnan Province 2007GH, National S&T Major Project 2009ZX09501-028, National Key Technology R&D Program 2007BAI33B00, and Chinese National Science Foundation 30700159 and 30700425.

语种：英文

外文关键词：Epithelial-mesenchymal transition; Rhesus monkey; Hepatic progenitor cells; TGF-beta; Myofibroblasts-like cells

外文摘要：The origin of the myofibroblast, the primary effector cell of liver fibrosis, is still elusive. Here, we report that fluorescence-activated cell sorting purified E-cad + rhesus monkey liver epithelial progenitor cells (mLEPCs) may serve as a potential source for liver myofibroblasts. Adult mLEPCs colonies were cultured in medium containing 2 ng/ml transforming growth factor beta (TGF-beta) and 10% fetal bovine serum (FBS) to induce differentiation. Phenotypic changes of cells were analyzed by morphological observation, immunostaining, and reverse transcription-polymerase chain reaction (RT-PCR). After cultured with TGF-beta and FBS, some cells in adult mLEPCs colonies converted to fibroblasts-like cells. Immunostaining showed that fibroblasts-like cells had acquired the expression of mesenchymal cell marker vimentin but lost the expression of epithelial cell marker CK8. Fibroblasts-like cells were maintained in culture for up to 40 passages. RT-PCR analysis revealed that fibroblasts-like cells had acquired the expression of mesenchymal genes (snail, PAI-1, and collagen I) and lost the expression of epithelial specific genes (E-cad, ZO-1, CK18, and occludin). In addition, more than 60% of fibroblasts-like cells expressed myofibroblastic-related proteins such as alpha SMA, vimentin, and N-cad, which were not presented in mLEPCs. Furthermore, increased cell motility was also detected in these fibroblasts-like cells by time-lapse video observation. Our results demonstrate that hepatic epithelial progenitor cells, mLEPCs, transform to myofibroblast-like cells via epithelial-mesenchymal transition. This finding will facilitate understanding of the origin of myofibroblasts in liver fibrosis.