文献类型：期刊文献

英文题名：Antioxidation and Anti-Inflammatory Activity of Prussian Blue Nanozymes to Alleviate Acetaminophen-Induced Acute Liver Injury

作者：Feng, Qiang[1,2];Xu, Hongting[1];Pan, Xiaoyu[1,3];Geng, Siqi[4];Qian, Huifeng[1];Wang, Chujie[1];Li, Ying[1];Qin, Jiaying[1];Wu, Yiting[1];Zhou, Bangyi[1];Liu, Xiaoyu[1];Ye, Zhiqiu[1];Xu, Yingchun[5];Yu, Zhangsen[1]

机构：[1]Shaoxing Univ, Sch Med, Med Sci Res Ctr, Lab Nanomed, Shaoxing 312000, Zhejiang, Peoples R China;[2]Fujian Med Univ, Coll Clin Med Oncol, P, R China, Fuzhou 350122, Peoples R China;[3]Fujian Med Univ, Affiliated Hosp 1, Fuzhou 350122, Peoples R China;[4]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[5]Shaoxing Peoples Hosp, Breast & Thyroid Surg Dept, Shaoxing 312000, Zhejiang, Peoples R China

年份：2023

外文期刊名：ACS APPLIED NANO MATERIALS

收录：SCI-EXPANDED(收录号：WOS:000986554300001)、、EI(收录号：20232114121696)、Scopus(收录号：2-s2.0-85159613546)、WOS

基金：This work was financially supported by the project of Zhejiang Provincial Natural Science Foundation (LGF21H180005 and LTGY23H020002) , the Science and Technology Plan in Shaoxing City (2022A14026) , Zhejiang University Laboratory Work Research Project (YB202219) , College Students' Science and Technology Innovation Project of Zhejiang Province (2022R432A026) , Medical and Health Science and Technology Plan Project of Zhejiang Province (2021KY1159) , and Medical and Health Science and Technology Plan Project of Shaoxing (2020A13024 and 2020A13060) .

语种：英文

外文关键词：Prussian blue nanozymes; acetaminophen; APAP-induced DILI; reactive oxygen species; inflammation regulation

外文摘要：Acetaminophen (APAP), a common antipyretic and analgesic drug, is considered the most common cause of drug-induced liver injury (DILI). The mechanism of liver injury induced by APAP is mainly related to oxidative stress and inflammatory reaction. Herein, we report a simple and efficient one-step synthesis of Prussian blue (PB) nanozymes with multiple antioxidant enzymatic activities that effectively treat APAP-induced DILI. At the cellular level, reaching 10 mu g/mL PB nanozymes can effectively scavenge intracellular reactive oxygen species (ROS), reduce mitochondrial membrane potential drop, and inhibit hepatocyte apoptosis. According to in vivo experimental studies, the levels of serum biochemical indicators and histopathological examination of DILI mice livers showed that 12.5 mg/kg PB nanozymes could effectively inhibit liver necrosis and 25 mg/kg PB nanozymes achieved the same therapeutic effect as 300 mg/kg NAC. More importantly, compared with NAC, PB nanozymes can still attenuate APAP-induced acute liver injury in mice after APAP-induced acute liver injury in mice for 3 h. Therefore, PB nanozymes can effectively prolong the therapeutic time window, revealing the potential of PB nanozymes in clinical applications for advanced DILI treatment. Furthermore, the therapeutic mechanism studies have shown that PB nanozymes with abundant and variable valence states could not only directly scavenge ROS but also through the Keap1-Nrf2/HO-1 pathway to reduce oxidative stress. Moreover, the decreased expression levels of myeloperoxidase and F4/80 in the liver, which are markers of neutrophil and macrophage infiltration, indicated that the Prussian blue nanozymes modulates inflammation to protect against APAP-induced acute liver injury. Consequently, our findings suggested that PB nanozymes have excellent clinical application prospects for acetaminophen-induced acute liver injury.