文献类型：期刊文献

英文题名：Optimization of digested chicken manure filtrate supplementation for lipid overproduction in heterotrophic culture Chlorella protothecoides

作者：Liang, Guobin[1];Mo, Yiwei[2];Zhou, Quanfa[1]

机构：[1]Jiangsu Univ Technol, Sch Chem & Environm Engn, Changzhou 213001, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 321000, Peoples R China

年份：2013

卷号：108

起止页码：159

外文期刊名：FUEL

收录：SCI-EXPANDED(收录号：WOS:000317565100021)、、WOS

基金：The study acknowledged the support in grants from programs of ministry of Science and Technology of China (2008BAC46B04, 2011BAC10B03), education department of Jiangsu province (10KJB610002) and Jiangsu University of Technology (KYY09003).

语种：英文

外文关键词：Digested chicken manure filtrate (DCMF); Heterotrophic culture; Lipid production; Glucose; Chlorella protothecoides

外文摘要：This study was to investigate effects of digested chicken manure filtrate (DCMF) supplementation on cells growth and lipid production in heterotrophic culture Chlorella protothecoides. Higher nitrogen DCMF favored cells growth but retarded lipid accumulation and vice versa for lower nitrogen DCMF. Accordingly, a two-step DCMF supplementation method of feeding higher nitrogen DCMF at cell growth phase and lower one at the stationary phase was adopted to enhance lipid production. Moreover, based on the phenomenon that feeding glucose at the stationary phase can further enhance lipid production, the strategies of DCMF supplementation coupled with adenosine triphosphate (ATP) regeneration from glucose feeding were developed. As a result, total lipid yield of 5.28 g/L was achieved after 200 h cultivation, increased by 74.8% and 21.1% respectively, compared to the control and the one without glucose addition. It was suggested that application of DCMF combined with glucose feeding as being feasible for lipid overproduction. (C) 2013 Elsevier Ltd. All rights reserved.