文献类型：期刊文献

中文题名：一株产吲哚乙酸耐盐促生菌的分离、鉴定及发酵条件优化

英文题名：Isolation, identification and fermentation conditions optimization of a salt-tolerant,growth-promoting and indoleacetic acid-producing bacterium

作者：贾西贝[1];王琦琦[1];李杨[1];褚贵新[2];孙燕飞[1]

机构：[1]石河子大学生命科学学院,新疆石河子832003;[2]绍兴文理学院环境科学与工程系,浙江绍兴312000

年份：2019

卷号：38

期号：11

起止页码：37

中文期刊名：中国酿造

外文期刊名：China Brewing

收录：CSTPCD、、北大核心2017、北大核心

基金：国家重大基础研究专项新疆干旱区盐碱地生态治理关键技术研究与集成示范(2016YFC0501404)

语种：中文

中文关键词：菌株筛选;鉴定;吲哚乙酸;培养基成分;发酵条件;优化

外文关键词：strain screening;identification;indoleacetic acid;medium composition;fermentation condition;optimization

中文摘要：从碱蓬根际土壤中分离筛选获得一株能产吲哚乙酸(IAA)的促生菌株BG-5,对该菌株进行了16S rDNA序列分析。以IAA的产量作为评价指标,利用单因素试验及响应面分析法对发酵条件及培养基成分进行优化。结果表明,BG-5菌株被鉴定为短小芽孢杆菌(Bacillus pumilus),最佳培养条件为装液量30 mL/250 mL、初始pH值为8、培养温度37℃;优化后的培养基成分为酵母提取物7.3 g/L,胰蛋白胨8.8 g/L,七水硫酸镁7.2 g/L;在此最优条件下,BG-5菌株的吲哚乙酸产量为87.86μg/mL,约为优化前的2倍。

外文摘要：A growth-promoting and indoleacetic acid(IAA)-producing strain BG-5 was isolated and screened from the rhizosphere soil of Suaeda salsa,and analyzed by 16S rDNA sequences.Using the yield of IAA as evaluation index,the fermentation conditions and medium composition were optimized by single factor tests and response surface methodology.The results showed that the strain BG-5 was identified as Bacillus pumilus.The optimal culture conditions were loading volume 30 ml/250 ml,initial pH 8 and culture temperature 37℃.The optimal medium composition was yeast extract 7.3 g/L,tryptone 8.8 g/L,and magnesium sulfate heptahydrate 7.2 g/L.Under the optimal conditions,the IAA yield of the strain BG-5 was 87.86μg/ml,which was about twice that of before optimization.