文献类型：期刊文献

中文题名：PCNA和C-fos蛋白在人胎食管组织中的表达及意义

英文题名：Expression of PCNA and C-fos proteins in the human fetal esophagus

作者：刘学红[1];张泳[1]

机构：[1]绍兴文理学院医学院

年份：2010

期号：34

起止页码：3690

中文期刊名：世界华人消化杂志

外文期刊名：World Chinese Journal of Digestology

收录：Scopus、北大核心2008、北大核心

基金：浙江省教育厅科研计划基金资助项目;No.Y200805569;绍兴文理学院重点基金资助项目;No.08LG1018~~

语种：中文

中文关键词：食管;增殖细胞核抗原;C-fos蛋白;人胚胎;免疫组织化学

外文关键词：Esophagus; Proliferating cell nuclear antigen; C-fos protein; Human fetus; Immunohisto-chemistry

中文摘要：目的:探讨增殖细胞核抗原(PCNA)和C-fos蛋白在人胚胎食管组织中的表达规律.方法:应用免疫组织化学PV法、SP法和图像分析(NIS-DR)软件检测第2、3、4月,人胚胎食管组织中PCNA和C-fos蛋白阳性表达的积分光密度(IA),数据的组间比较采用单因素方差分析.结果:第2月胚龄组食管组织中,PCNA蛋白阳性细胞的IA值为50.6540±5.92400(n=5),3mo胎龄组IA值为57.8465±9.99749(n=6),4mo胎龄组IA值为69.3626±16.28008(n=5),可见IA值依次增高,两两比较,差异有统计学意义(P<0.01).第2、3、4个月胎龄段,C-fos蛋白在人胚胎食管各层组织细胞均呈阳性表达.2mo胎龄组食管组织中,C-fos蛋白阳性染色细胞的IA值为77.7867±12.22991(n=5),3mo胎龄组IA值为83.2524±8.63989(n=6),4mo胎龄组IA值为66.5414±9.06673(n=5),可见IA值先升高再降低,两两比较,差异有统计学意义(P<0.01).结论:PCNA和C-fos蛋白在人胚胎早期食管组织细胞的生长发育过程中起重要的调节作用.

外文摘要：AIM：To investigate the expression pattern of proliferating cell nuclear antigen（PCNA）and c-Fos proteins in the human fetal esophagus.METHODS：The expression of PCNA and c-Fos in the esophagus tissue samples taken from hu-man fetus at 2,3 or 4 months was detected by immunohistochemistry.The integral absorbance（IA）values of PCNA and C-fos staining were calculated using the Nikon imaging system（NIS-DR）.One-way ANOVA was applied to compare these IA values.RESULTS：Positive PCNA protein expression was detected in all the layers of the esophagus of human fetus at 2,3 or 4 months.The IA value of PCNA-positive cells in the esophagus of human fetus at 2 months was significantly lower than those at 3 and 4 months（50.6540±5.92400 vs 57.8465±9.99749 and 69.3626±16.28008,both P 0.01）.With the increase in fetal age,the IA val-ues of PCNA-positive cells showed an upward trend.Positive expression of C-fos protein was also detected in all the layers of the esophagus of human fetus at 2,3 or 4 months.The IA value of C-fos-positive cells in the esophagus of hu-man fetus at 3 months was significantly higher than those at 2 and 4 months（83.2524±8.63989 vs 77.7867±12.22991,66.5414±9.06673,both P 0.01）.With the increase in fetal age,the IA value of C-fos-positive cells rose initially and then dropped.CONCLUSION：PCNA and C-fos proteins play an important role in regulating the growth and development of the human fetal esophagus.