文献类型：期刊文献

英文题名：The dual role of eppin in immunity and biomineralization during nacreous layer formation in mollusks

作者：Jin, Can[1];Jiang, Rui[1];Zhang, Yihang[1];Cheng, Kang[1];Luo, Wen[1];Xie, Guilin[2]

机构：[1]Shaoxing Univ, Sch Life & Environm Sci, Shaoxing 312000, Peoples R China;[2]Shaoxing Univ, Dept Hepatobiliary Surg, Affiliated Hosp, Shaoxing 312000, Peoples R China

年份：2023

卷号：25

期号：36

起止页码：5160

外文期刊名：CRYSTENGCOMM

收录：SCI-EXPANDED(收录号：WOS:001055805600001)、、EI(收录号：20233714721655)、Scopus(收录号：2-s2.0-85170526766)、WOS

基金：This work was supported by the National Natural Science Foundation of China (32202921) and the Sci-tech Special Commissioners Project of Zhejiang Province-Pearl Quality Improvement and Industrialization (2020-2024).

语种：英文

外文关键词：Acceleration - Bacteria - Calcium carbonate - Deposition - Gems - Molluscs - Shells (structures)

外文摘要：The presence of protease inhibitors in a carbonated shell is intriguing and opens questions about their potential role in shell biomineralization. Our previous proteome study detected an epididymal protease inhibitor (eppin) which was strongly expressed in pearl nacre. Herein, we obtained the full-length complementary DNA sequence of eppin from Hyriopsis cumingii. The expression of eppin was localized to the dorsal epithelial cells of the mantle pallium and mantle center and significantly increased during pearl nacre deposition and shell nacreous layer regeneration. These results indicated that eppin is essential for nacre biomineralization. The expression of eppin was significantly increased at 3 h after Staphylococcus aureus challenge. GST-eppin could significantly inhibit the growth of S. aureus and effectively inhibit chymotrypsin activity. Inhibition of eppin in extrapallial fluid induced haphazard distribution of nanoscale grains and the destruction of the organic framework of the nacreous layer. These results indicated that eppin had a long-lasting antibacterial activity and provided a matrix protecting system that prevented extracellular degradation of matrix proteins. In addition, GST-eppin affected morphology regulation and induced the acceleration of calcium carbonate deposition. These results added to our understanding of the mode of action of protease inhibitors and their roles in shell biomineralization. The presence of protease inhibitors in a carbonated shell is intriguing and opens questions about their potential role in shell biomineralization.