文献类型：期刊文献

英文题名：Overexpression of CBL interacting protein kinase 2 improves plant tolerance to salinity and mercury

作者：Pan, W. H.[1];Zheng, Z. Z.[2];Yan, X.[2,3];Shen, J. Q.[2];Shou, J. X.[1];Jiang, L. X.[4];Pan, J. W.[3]

机构：[1]Shaoxing Univ, Coll Life Sci, Shaoxing, Zhejiang 312000, Peoples R China;[2]Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua, Zhejiang 321004, Peoples R China;[3]Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China;[4]Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China

年份：2019

卷号：63

起止页码：183

外文期刊名：BIOLOGIA PLANTARUM

收录：SCI-EXPANDED(收录号：WOS:000466354600023)、、Scopus(收录号：2-s2.0-85067545291)、WOS

基金：We thank Dr. G. Zhang (Zhejiang University, China) and Dr. Y. Yang (Pennsylvania State University, USA) for generously sharing published materials. This work was supported by the China National Key Programs for Transgenic Crops (No. 2009ZX08009-076B), the National Natural Science Foundation of China (Nos. 31670283 and 91754104), and the Natural Science Foundation of Zhejiang Province (Nos. LY17C060001, LY16C130004, and Y3100207). The first three authors contributed equally to this work.

语种：英文

外文关键词：cadmium; copper; gene expression; NaCl; root growth; seed germination; subcellular localization

外文摘要：In plants, calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) regulate Ca2+ signalling and so responses to biotic and abiotic stresses. However, the details of specific CIPKs functions in various stress responses are poorly understood. Here, we report roles of dicot and monocot CIPK2 genes in response to salinity and heavy metals. Arabidopsis thaliana AtCIPK2 was found to be universally expressed in different tissues and organs and furthermore induced by salinity. Overexpression of AtCIPK2 or Tibetan Plateau wild barley (Hordeum spontaneum) HsCIPK2 in Arabidopsis alleviated toxic effects of NaCl and mercury on seed germination and root growth. Similarly, reduced toxic effects of copper and cadmium on seed germination, but not on root growth, were observed in these transgenic lines. Live-cell fluorescence imaging analysis revealed that HsCIPK2 was predominantly distributed in the cytoplasm and nucleus and weakly localized at the plasma membrane (PM), but its PM association was rapidly enhanced upon exposure to high salinity and mercury. These results suggest an involvement of CIPK2 in plant tolerance to salinity and mercury and provide a new insight into physiological functions of CIPKs in plant response to heavy metals.