文献类型：期刊文献

英文题名：Attenuation of hepatic fibrosis through ultrasound-microbubble-mediated HGF gene transfer in rats

作者：Jiang, Zhen-zhen[1,2];Xia, Guo-yuan[1];Zhang, Yong[1];Dong, Liang[1];He, Ben-zhen[2];Sun, Jian-gang[1]

机构：[1]Shaoxing Univ, Sch Med, Shaoxing 312000, Zhejiang, Peoples R China;[2]Wenzhou Med Coll, Clin Med Inst 1, Wenzhou, Zhejiang, Peoples R China

年份：2013

卷号：37

期号：1

起止页码：104

外文期刊名：CLINICAL IMAGING

收录：SCI-EXPANDED(收录号：WOS:000312284100015)、、EI(收录号：20130415928062)、Scopus(收录号：2-s2.0-84872390153)、WOS

基金：This work was supported by Zhejiang Scientific Technology Foundation (No. Y2110966) and Zhejiang Natural Science Foundation (No. 2008C33057).

语种：英文

外文关键词：Hepatic fibrosis; Gene transfer; Ultrasound; CCl4

外文摘要：Objective: The objective was to explore the feasibility or ultrasound-microbubble-mediated hepatocyte growth factor (HGF) gene transfer for treating rat hepatic fibrosis induced by CCl4. Methods: Forty-eight male SD rats were divided into ultrasound-microbubble-HGF group (U-M-HGF group), ultrasound-HGF group (U-HGF group), microbubble-HGF group (M-HGF group), HGF group (HGF group), CCl4 group (control group), and normal group. The serum levels of alanine transaminase (ALT), aspartate transaminase (AST), total protein, albumin (ALB), and globulin (GLB) and the ratio of ALB/GLB were determined after treatment. The degree of hepatic fibrosis was evaluated by histopathological numerical scores. The protein expressions of HGF, collagen I, collagen III, and alpha-smooth muscle antibody (alpha-SMA) were detected by immunohistochemistry. Results: Ultrasound-microbubble-mediated HGF therapy significantly reduced the serum level of ALT and AST to 59.88% and 49.18% of the control group, respectively. Ultrasound-microbubble-mediated HGF therapy prevented liver fibrosis, with an obvious decrease in fibrosis areas and extracellular matrix production of collagen I, collagen III, and alpha-SMA. The gene therapy could induce HGF delivery into the fibrotic liver effectively. Conclusions: Ultrasound-microbubble-mediated HGF gene therapy can reduce liver fibrosis, which provides a novel strategy for gene therapy of chronic liver disease. (C) 2013 Elsevier Inc. All rights reserved.