文献类型：期刊文献

中文题名：西酞普兰对慢性应激大鼠额叶皮质神经细胞bax mRNA、bcl-2 mRNA表达及凋亡的影响

英文题名：Effects of Citalopram on frontal cortical neurons' bax mRNA、bcl-2 mRNA expression and cell apoptosis of rat after stress

作者：俞爱月[1];孙小红[1];刘学红[2];周瑾[2];王岚[2]

机构：[1]绍兴文理学院元培学院;[2]绍兴文理学院医学院

年份：2015

卷号：31

期号：5

起止页码：455

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：MEDLINE(收录号：26827541)、CSTPCD、、CSCD2015_2016、Scopus、CSCD、PubMed

基金：浙江省绍兴市2010重点科研项目(2010A23019)

语种：中文

中文关键词：西酞普兰;慢性应激;bax;mRNA;bcl-2;mRNA;细胞凋亡;额叶皮质;大鼠

外文关键词：Citalopram; chronic stress; bax mRNA; bcl-2 mRNA; apoptosis; prefrontal cortex; rat

中文摘要：目的:探讨西酞普兰对慢性应激大鼠的额叶皮质神经细胞bax、bcl-2 mRNA表达的影响。方法:取24只雄性SD大鼠随机分为对照组(不进行任何处理)、应激组(应激+生理盐水灌胃)、实验组(应激+西酞普兰灌胃),采用强迫游泳制造慢性应激模型(15 min/d,共4周),用原位杂交技术检测bax、bcl-2 mRNA表达情况,TUNEL法检测细胞凋亡,尼康图像分析(NIS DR)软件测量各指标阳性细胞数量。结果:应激组较对照组,大鼠额叶皮质bax mRNA阳性表达细胞明显增多(P<0.01)、染色加深;bcl-2 mRNA阳性表达细胞明显减少(P<0.01)且染色变浅,且TUNEL阳性细胞数量增多(P<0.01),染色增强。而实验组较应激组大鼠,额叶皮质bax mRNA阳性表达细胞减少(P<0.01)、染色变浅,bcl-2 mRNA阳性表达细胞明显增多(P<0.05),染色加深,且TUNEL阳性细胞数量减少(P<0.01),染色减弱。结论:大鼠额叶皮质神经细胞bax mRNA和bcl-2 mRNA的表达水平受到慢性应激的影响,导致细胞凋亡加剧,西酞普兰能够有效调控额叶皮质神经细胞bax和bcl-2 mRNA的表达水平,拮抗细胞凋亡,这可能是西酞普兰防治慢性应激引起的神经精神疾病的相关机制之一。

外文摘要：Objective： To study the effects of Citalopram on the mRNA expression of bax and bcl-2 in frontal cortical neurons and on cell apoptosis of rats after stress. Methods： Twenty-four healthy male SD rats were randomly divided into three groups （ n = 8）. The control group did no receive any treatment, the stress group was subject to stress and given normal saline and experimental group was given Citalopram irriga- tion stomach after stress. Rats were forced to swim to establish chronic stress model （15min/d,4 weeks）, bax,bcl-2 mRNA expression were tested by in situ hybridization technique （ISH）, TUNEL assay was used to determine cell apoptosis , Nikon image analysis software were used to measure the number of positive cells in each index. Results： Compared with the control group, the stress group showed a larger number of bax mRNA expressing cells（ P 〈 0.01）, a smaller number of bcl-2 mRNA expressing cells （ P 〈 0.01）, and the staining intensity of positive cells was significantly reduced（ P 〈 0.01）. Compared with the stress group, the experiment group showed more reduced number of bax mRNA positive cells（ P 〈 0.01 ）and significantly increased bcl-2 mRNA positive cells（ P 〈 0.05） , a small amount of positive cells were found, com- pared with that in the stress group, nuclear condensation in the experimental group was reduced significantly and the staining was obviously weaker（ P 〈 0.01）. Conclusion： Citalopram significantly antagonizes bax mRNA and potentiatesbcl-2 mRNA protein expression and inhibits apeptosis of rat prefrontal cortical neurons caused by chronic stress, which might be one possible mechanism of Citalopram for prevention and treatment of psychosis caused by chronic stress.