文献类型：期刊文献

中文题名：福寿螺SOX9L基因的克隆与表达分析

英文题名：Cloning and Expression Analysis of SOX9L Gene in Pomacea canaliculata

作者：张爽[1];苏靖景[1];王欣慧[1];张颖[1];刘丰瑜[1];刘婧琪[1];袁紫霞[1];吕佳励[1];商凯琳[1];杨受保[1]

机构：[1]绍兴文理学院生命科学学院,绍兴312000

年份：2023

卷号：31

期号：9

起止页码：1914

中文期刊名：农业生物技术学报

外文期刊名：Journal of Agricultural Biotechnology

收录：CSTPCD、、CSCD2023_2024、北大核心、CSCD、北大核心2020

基金：绍兴文理学院中青年学术骨干培养对象项目(2014-19);绍兴市科技局公益项目(2018C20012);浙江省大学生新苗人才计划(2022R432A006);国家级大学生创新创业训练计划(202110349046X)。

语种：中文

中文关键词：福寿螺;SOX9(SRY-related HMG box gene 9);胚胎发育;性别决定

外文关键词：Pomacea canaliculata;SRY-related HMG box gene 9(SOX9);Embryonic development;Sex determination

中文摘要：福寿螺(Pomacea canaliculata)是中国的16种危害最大的外来入侵物种之一。SOX9(SRY-related HMG box gene 9)是一类与早期胚胎发育有关的SOXE亚族基因。为探究福寿螺SOX9基因的功能,以不同发育阶段的福寿螺—仔螺(rank Ⅱ)、中螺(rank Ⅲ)和雌雄成螺(rank Ⅳ)为材料,在转录组测序的基础上,采用RT-PCR技术克隆得到福寿螺SOX9类似物基因的cDNA序列,命名为PcSOX9L(GenBank No.OP564918),并对其进行生物信息学和表达特性分析。结果表明,PcSOX9L基因包含1个1 701 bp的开放阅读框,编码566个氨基酸的假定蛋白,并含有2个保守结构域,分别是Sox-N和HMG-box-domain;PcSOX9L与其他动物SOX9蛋白的氨基酸序列相似性为52.72%~82.84%。qRT-PCR分析显示,PcSOX9L基因在雌雄成螺的性腺、肝脏、鳃、外套膜、腹足和雌性成螺的蛋白腺组织中均有表达,其中,在雄性成螺肝脏中表达量最高,且极显著高于雌性成螺的肝脏组织(P<0.01);在性腺组织中,雄性成螺的精巢比雌性成螺卵巢中的表达量高,但是差异不显著;PcSOX9L在从仔螺、中螺到性成熟成螺的3个不同发育阶段中均有表达,在仔螺中表达量最高;同时,PcSOX9L表达量在向雄性成螺发育的3个阶段中依次为仔螺>雄性成螺>中螺;在向雌性成螺发育的3个阶段中依次为仔螺>中螺>雌性成螺,提示该基因可能在福寿螺早期胚胎发育和性别决定中发挥作用。本研究为进一步分析SOX9基因在福寿螺性腺发育和性别决定中的作用机制提供基础资料。

外文摘要：Pomacea canaliculata is one of the 16 most harmful invasive alien species in China.SRY-related HMG box gene 9(SOX9)is a subgroup of SOXE genes associated with early embryonic development.In order to explore the function of the SOX9 gene of snails,the cDNA sequence of the SOX9 like gene from snail P.canaliculata(named as PcSOX9L,GenBank No.OP564918)was cloned by RT-PCR on the basis of transcriptome sequencing.Using P.canaliculata of different developmental stages(rankⅡ~ⅣP.canaliculata)as materials,the bioinformatics as well as expression characteristics of PcSOX9L were analyzed.The results showed that PcSOX9L gene contained a 1701 bp open reading frame,encoding a hypothetical protein of 566 amino acids,and contained 2 conserved domains,namely Sox-N and HMG-box-domain;the amino acids of PcSOX9L and other animal SOX9 proteins showed that the sequence similarity ranged from 52.72%to 82.84%.qRT-PCR analysis showed that PcSOX9L gene was expressed in various tissues including gonad,liver,gill,mantle,and pleopod of rankⅣP.canaliculata,as well as the albumin gland of female rankⅣP.canaliculata.Among them,the expression level of PcSOX9L was the highest in the liver of male rankⅣP.canaliculata,and was extremely significantly higher than the same tissues of female rankⅣP.canaliculata(P<0.01).No significant difference was detected between the gonad of male and female rankⅣP.canaliculata.The PcSOX9L was expressed in 3 different developmental stages,with the highest expression in rankⅡP.canaliculata;at the same time,the changes of PcSOX9L expression in 3 different developmental stages toward male rankⅣP.canaliculata in descending order as rankⅡP.canaliculata>male rankⅣP.canaliculata>>rankⅢP.canaliculata;and the changes of PcSOX9L expression in 3 different developmental stages toward female rankⅣP.canaliculata in descending order as rankⅡP.canaliculata>rankⅢP.canaliculata>female rankⅣP.canaliculata,which indicated that this gene might play an important role in the early embryonic development and sex determination of P.canaliculata.The present results provide basic data for further analysis of the mechanism of SOX9 gene in the gonad development and sex determination of P.canaliculata.