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绍兴黄酒酒药中酵母菌的物种资源     被引量:17

Yeast species in the fermentation starter of Shaoxing ricewine

文献类型:期刊文献

中文题名:绍兴黄酒酒药中酵母菌的物种资源

英文题名:Yeast species in the fermentation starter of Shaoxing ricewine

作者:臧威[1];谢广发[2,3];孙剑秋[1];邹慧君[2,3];刘新展[4];钱斌[2,3];沈国娟[1];白逢彦[4]

机构:[1]绍兴文理学院生命科学学院;[2]国家黄酒工程技术研究中心;[3]浙江古越龙山绍兴酒股份有限公司;[4]中国科学院微生物研究所真菌学国家重点实验室

年份:2015

卷号:34

期号:6

起止页码:1078

中文期刊名:菌物学报

外文期刊名:Mycosystema

收录:CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金:中国科学院微生物研究所真菌学国家重点实验室开放课题(SKLMKF201307);浙江省重大科技专项(2013C02006-1);绍兴市公益性技术应用研究计划(2014B70042)~~

语种:中文

中文关键词:酵母菌;酒药;绍兴黄酒;扣囊复膜孢酵母

外文关键词:yeast, jiuyao, Shaoxing ricewine, Saccharomycopsisfibuligera

中文摘要:酒药是绍兴黄酒酿造中使用的重要糖化发酵剂。2013年绍兴黄酒冬酿期间,从绍兴地区5个主要黄酒生产企业,采集古越龙山、会稽山、塔牌、沈永和、女儿红及鉴湖等6个品牌酒药样本,在数量、种类和分布规律等方面对黄酒酒药内酵母菌物种资源进行了系统研究。研究发现,会稽山酒药和沈永和酒药蕴含酵母菌数量较多而且与其他样本相比差异显著(P<0.01),而塔牌酒药中酵母菌数量显著少于其他5个品牌(P<0.05)。遵循酵母菌分类原则,从绍兴黄酒酒药中分离鉴定出3种酵母菌,扣囊复膜孢酵母Saccharomycopsis fibuligera、酿酒酵母Saccharomyces cerevisiae和隐球酵母Cryptococcus sp.。其中,扣囊复膜孢酵母Saccharomycopsis fibuligera在6个品牌黄酒酒药内的分布占有绝对优势,该种酵母菌可能对绍兴黄酒酒药的生产性能来说至关重要。比较分析酒药中酵母菌物种资源,可以为阐明绍兴黄酒的发酵机制提供基础数据和理论基础。

外文摘要:"jiuyao" is an important saccharification and fermentation starter used in the production of Shaoxing ricewine Samples of starter (jiuyao) used for the fermentation of different brands of Shaoxing ricewine, including Guyuelongshan, Kuaijishan, Tapai, Shenyonghe, Nuerhong and Jianhu, were collected during the winter of 2013 and then subjected for quantitative yeast isolation. The result showed that the colony counts of yeasts in the starter samples of Kuaijishan and Shenyonghe were significantly higher than those in the other samples (P〈O.01), while the Tapai samples showed the lowest yeast counts among the samples compared (P〈0.05). Three yeast species, Saccharomycopsis fibuligera, Soccharomyces cerevisiae and Cryptococcus sp., were identified from the yeast strains isolated using the 26S rDNA D1/D2 domain sequence analysis. Saccharomycopsis fibuligera was found to be an dominant species in all the samples examined, suggesting that this species may be crucial for the quality of starter.

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