文献类型：期刊文献

中文题名：少突胶质细胞转录因子和神经钙黏附蛋白在人胚胎脊髓发育阶段的表达

英文题名：Expression of oligodendrocyte transcription factor and neurological cadherin in the developing spinal cord of the human embryo

作者：张泳[1];张居适[2];陈健尔[1];刘学红[1]

机构：[1]绍兴文理学院医学院;[2]绍兴市中医院

年份：2015

卷号：46

期号：5

起止页码：655

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、北大核心2014、CSCD2015_2016、Scopus、北大核心、CSCD

基金：浙江省自然科学基金资助项目(LY15H170001);绍兴市公益性技术应用研究计划资助项目(2013D10035);浙江省公益性技术应用研究计划资助项目(2012C33066)

语种：中文

中文关键词：胚胎;脊髓;少突胶质细胞转录因子;神经钙黏附蛋白;免疫组织化学;人

外文关键词：Embryo; Spinal cord; Oligodendrocyte transcription factor; Neurological cadherin;Immunohistochemistry ; Human

中文摘要：目的 探讨少突胶质细胞转录因子（Olig）和神经钙黏附蛋白（N-cadherin）在人胚胎脊髓发育阶段的分布规律及其表达意义。方法 应用免疫组织化学法检测第2、3、4月龄段,共17例人胚胎,脊髓中Olig和N-cadherin的表达和分布状况。应用One-Way ANOVA和LSD-t统计学方法,分析第2-4个月龄段人胚胎脊髓组织内N-cadherin和Olig蛋白的各自阳性表达强度值及Olig蛋白的阳性细胞表达数量。结果 第2-4个月龄段,随着胎龄的增大,N-cadherin在脊髓组织的阳性表达强度值逐渐增大,其值分别是117.77±6.72、129.63±9.44和133.35±8.15,差异有统计学意义（P〈0.01）;Olig阳性表达强度值呈先升高再降低,其值分别是69.98±24.59、85.10±9.19、71.62±11.81,差异有统计学意义（P〈0.05）;Olig阳性表达细胞数量逐渐增多,其值分别是9.38±7.80、15.55±7.30、25.59±14.54,差异有统计学意义（P〈0.01）。结论 N-cadherin和Olig在第2-4个月龄段人胚胎脊髓组织内均有阳性表达,提示其表达分布与脊髓组织内神经细胞和神经胶质细胞的分化、发育关系密切。

外文摘要：Objective To investigate distribution of the oligodendrocyte transcription factor （Olig） and neurological cadherin（N-cadherin） in the developing human embryonic spinal cord and their clinical significance. Methods Immunohistochemistry was uesd to detect the expression and distribution of Olig and N-cadherin proteins in the spinal cord of 17 human embryos at the second, third or fourth month gestation. One-Way ANOVA and LSD-t test were employed to compare positive expression number and intensity values of Olig and N-cadherin proteins in spinal cord tissues detected in the 3 fetal periods. Results At the second to fourth gestation, the positive expressions of Olig and N-cadherin were seen in the human embryonic spinal cord. With the increase of gestational age, the intensity values of N-cadherin positive cells were gradually increased in the spinal cord, and positive expression intensity values were 117.77 ± 6.72,129.63 ± 9.44 and 133.35± 8.15 （P 〈0. 01 ） , respectively. Olig positive expression intensity values showed an up and then down trend were 69.98 ±24.59,85.10± 9. 19 and 71.62 ± 11.81 （ P 〈 0. 05 ） , respectively. The number of Olig positive ceils in the spinal cord were gradually increased and were 9.38 ± 7.80,15.55 ± 7.30,25.59 ± 14.54 （ P 〈 0. 01 ） , respectively. The results of Olig and N-cadherin showed a significant difference in the spinal cord. Conclusion At the second to fourth gestation, the positive expressions of N-cadherin and Olig suggest that they are closely related to the nerve and glial ceils differentiation and development in the spinal cord tissues during the human embryonic growth and development.