文献类型：期刊文献

中文题名：西酞普兰对慢性应激大鼠海马CA1、CA3区神经细胞凋亡的影响

英文题名：Preventive function of citalopram on neuro-cell apoptosis caused by long-term stress in CA1 and CA3 region of hippocampus

作者：俞爱月[1];苏巧荣[1];刘学红[2];王岚[3];张剑[4]

机构：[1]绍兴文理学院医学院心理学教研室;[2]绍兴文理学院医学院组织胚胎学教研室;[3]绍兴文理学院医学院基础医学实验中心;[4]绍兴文理学院医学院药理教研室

年份：2008

卷号：24

期号：8

起止页码：1557

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：浙江省绍兴市2007重点科研资助项目(No.2007A23005)

语种：中文

中文关键词：细胞凋亡;应激;海马;西酞普兰

外文关键词：Apoptosis ; Stress ; Hippocampus ; Citalopram

中文摘要：目的:探讨西酞普兰对慢性应激大鼠海马CA1、CA3神经细胞凋亡的影响。方法:将40只雄性SD大鼠随机分为空白、对照(生理盐水灌胃)、实验1-3组(分别以8mg·kg-1.d-1、4mg·kg-1.d-1、1mg.kg-1.d-1氢溴酸西酞普兰灌胃),每组8只,采用强迫游泳制造慢性应激大鼠模型,用大鼠悬尾实验、力竭实验观察行为学,苏木素伊红(HE)染色观察CA1、CA3神经细胞凋亡,脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)检测和尼康图像分析(NISBR)软件测量CA1、CA3神经细胞凋亡数量及积分吸光度值。结果:对照组静止不动时间延长、挣扎次数减少,实验组静止不动时间缩短、力竭时间延长、挣扎次数增多;对照组CA1、CA3区阳性细胞增多,CA3区阳性细胞积分吸光度值变小;各实验组阳性细胞数量减少,实验1、3组CA1、CA3区阳性细胞积分吸光度值增大。结论:西酞普兰对慢性应激大鼠海马CA1、CA3神经细胞具有保护作用,提示西酞普兰对慢性应激引起的神经精神疾病的治疗机制,可能是通过拮抗CA1、CA3区神经细胞凋亡而起作用。

外文摘要：AIM ： To explore the preventing effect of citalopram on neuro - cell apoptosis caused by long - term stress in CA1 and CA3 region of hippocampus. METHODS ： Forty male Sprague Dawley （SD） rats were randomly divided into five groups including blank group, control group （ the control group was filled the stomade by 0. 9% saline） and three experimental groups （intragastric administration of citalopram hydrobromide at doses of 8 mg·kg^-1·d^-1,4 mg·kg^-1·d^-1 , 1 mg·kg^-1·d^-1 , respectively）. Rat stress model was made by compulsory swimming everyday for 4 weeks. Cell apoptosis in CA1 and CA3 region of hippocampus was observed by HE staining method. Apoptotic cell numbers and integral optical density in CA1 and CA3 region were tested and analyzed by terminal deoxynucleotidyl transferase biotin - dUTP nick end labeling （TUNEL） method and Norton Internet Security BR （NIS BR） Software. t -test was applied to compare apoptosis cell numbers and integral optical density. RESULTS： Control rats showed more static time and less struggling times. Conversely, static time was shorter and rats spent more time after exhaustive exercise, and more struggling times in the experimental group. Rats in control group showed more positive cells in CA1 and CA3 regions and higher integral optical den- sity in CA3 region than those in blank group. Rats in experimental groups showed fewer positive cells in CA1 and CA3 regions. Rats in experimental group 1 and group 3 showed higher integral optical density in CA1 and CA3 regions than that in control group. CONCLUSION： Long - term stress might cause neuro - cell apoptosis in CA1 and CA3 region of hippocampus. Citalopram might have prophylactic effect on apoptosis caused by long - term stress in CA1 and CA3 region, and the prophylactic effect might not be influenced by citalopram. Our study suggests that the treatment mechanism of citalopram in neural and mental illness by long - term stress may involve in a major role by antagonizing neuronal apoptosis in both the CA1 and CA3.