文献类型：期刊文献

中文题名：麻疹病毒抗原性变异及免疫保护效果研究

英文题名：Study on antigen variation of measles virus and immunoprotecive efficacy

作者：张建华[1];卢亦愚[2];严菊英[2];邵传森[3];徐宝祥[2];陆群英[2];龚黎明[2];周敏[2]

机构：[1]绍兴文理学院医学院病原学教研室;[2]浙江省疾病预防控制中心;[3]浙江大学医学院

年份：2003

卷号：19

期号：8

起止页码：935

中文期刊名：中国公共卫生

外文期刊名：China Public Health

收录：CSTPCD、、北大核心2000、CSCD_E2011_2012、北大核心、CSCD

语种：中文

中文关键词：麻疹病毒;抗原性变异;中和抗体

外文关键词：measles virus;antigen variation;neutralization antibody

中文摘要：目的　了解浙江省麻疹野毒株的抗原性变异状况以及麻疹疫苗的免疫保护效果。方法　采用麻疹国际标准株Edmonston株、疫苗株沪191与近几年浙江省分离的麻疹野毒株 (浙 98- 5和浙 0 0 - 4 )分别制备免疫血清 ,与各毒株进行交叉中和试验 ,并分别对不同来源的人群血清测定中和抗体滴度。结果　麻疹野毒株浙 98- 5与疫苗株沪191和Edm株的抗原比分别为 3 0和 2 6 ,浙 0 0 - 4与上述两毒株的抗原比分别为 7 30和 5 6 5 ,其抗原性存在着明显的差异。麻疹疫苗初免儿童和麻疹急性期病人血清对麻疹野毒株的中和抗体几何平均滴度 (GMT)分别为 15 0 3和 5 0 4 ,明显低于疫苗株沪191,GMT分别为 6 8 12和 11 76。但健康人群血清对两毒株的中和抗体滴度无显著性差异。结论　浙江省麻疹野毒株已出现较明显的抗原性变异 ,现行麻疹疫苗的免疫保护效果也受到一定影响。

外文摘要：Objective To study the antigen variation of wild-type measled virus (MV) that circulated in Zhejiang province and immunoprotecive efficacy of measles vaccine. Methods The anti-measles virus neutralization (NT) antibody titers in the immune serum of rats which were obtained in the earlier wild-type measles virus (Edmonston strain) and the vaccine strain and the two field strains (Zhe 98-5 and Zhe 00-4) isolated recently wae measured in cross neutralization test,meanwhile NT antibody titers in the serum of population which obtained from different sources were also measured respectively. Results Antigenic ratios of Zhe 98-5 strain to Hu 191 strain and Edm strain were 3.0 and 2.6 respectively,while those of Zhe 00-4 strain to the two standard strains described above were 7.30 and 5.65,suggesting an obvious mutual difference of antigenicity.The anti-wild type measles virus NT antibody geometric mean titer (GMT) in the serum of initial vaccines and naturally infected individuals were 15.03 and 5.04 respectively,which was obviously lower than anti-measles vaccine strain (GMT was 68.12 and 11.76 respectivety).But NT antibody titers to various strains in the serum of healthy population were with non-significant difference. Conclusion Antigenic variation of wild-type measles virus that circulated in Zhejiang presented obviouly and immunoprotecive effcacy of measles vaccine was partly influenced.