文献类型：期刊文献

英文题名：Adaptor protein-2 regulate root cell division and differentiation in Arabidopsis thaliana

作者：Hu, Tianwei[1];Zhu, Mingsong[1];Hu, Yibo[1];Zhang, Chi[1];Linghu, Yuting[1];Li, Huiqiang[3];Wang, Chao[1,2]

机构：[1]Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Peoples R China;[2]Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Zhejiang, Peoples R China;[3]Henan Agr Univ, Coll Life Sci, Zhengzhou 450046, Henan, Peoples R China

年份：2025

卷号：358

外文期刊名：PLANT SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001501403500001)、、Scopus(收录号：2-s2.0-105005862147)、WOS

基金：We thank Prof. Ding Zhaojun (Shandong University) for the markers: WOX5pro:GFP , QC25 , PLT2pro:CFP , PLT1pro:PLT1-YFP , PLT2pro:PLT2-YFP , SHRpro:SHR-GFP , and SCRpro:SCR-GFP . This work was supported by the National Natural Science Foundation of China (Nos. 31820103008) , the China Postdoctoral Science Foundation (2023M731462) , the Fundamental Research Funds for the Central Universities (lzujbky-2020-it13) and the foundation of the Key Labora-tory of Cell Activities and Stress Adaptations, Ministry of Education of Lanzhou University (lzujbky-2020-kb05) .

语种：英文

外文关键词：Clathrin; Adaptor protein-2; Auxin; Root stem cell; Cell division

外文摘要：In Arabidopsis, the root apical meristem consists of quiescent center (QC) and its surrounding stem cells, which form a stem cell niche (SCN) that develops into the root structure. The formation and maintenance of stem cells are regulated by hormones and transcription factors. Previous studies have shown that clathrin-mediated endocytosis impairs root growth; however, the underlying mechanisms remain unclear. Clathrin-mediated endocytosis is dependent on the function of clathrin, Adaptor Protein-2 (AP-2) and the TPLATE complex (TPC). In this study, our phenotypic analysis indicated that the reduced root length in the clathrin, AP-2, and TPC mutants resulted from a decreased number of meristem cells and a reduction in meristem size. Further modified pseudo-schiff propidium iodide (mPS-PI) staining revealed that clathrin heavy chain mutant, chc2-2, and ap2 sigma which is a subunit of AP-2 exhibited disordered columella stem cells (CSCs), decreased columella cells, abnormal cortex/endodermis initiation (CEI), and excessive division of the endodermis. Moreover, the transcription and protein levels of SHORTROOT (SHR) and SCARECROW (SCR) as well as PLETHORA1 (PLT1) and PLT2 were significantly decreased in ap-2 mutants. Yeast two-hybrid and bimolecular fluorescence complementation demonstrated that AP2 sigma interacted with SHR. In addition, disruption of AP-2 function reduced the auxin accumulation and the plasma membrane expression of its polar transporters PIN-FORMED1 (PIN1), PIN3 and PIN7. Taken together, this study revealed that AP-2 regulates both the SHR/SCR activity and the PINs-auxin transport to maintain the root division and differentiation in Arabidopsis thaliana.