文献类型：期刊文献

中文题名：亚洲百合金色号角组培快繁技术研究

英文题名：Study on Tissue Culture and Rapid Propagation of Lilium asiatic Hybrids Golden Horn

作者：林琼瑶[1];俞俐如[1];潘咪咪[1];邵胜男[1];曾康[1];孙小红[1];王国夫[1]

机构：[1]绍兴文理学院元培学院生命科学系

年份：2013

期号：2

起止页码：15

中文期刊名：园艺与种苗

外文期刊名：Horticulture & Seed

基金：绍兴市2011年科技计划项目(2011A22009);2012年度绍兴市大学生科技创新项目;浙江省大学生科技创新活动计划项目(2012R426034)

语种：中文

中文关键词：亚洲百合金色号角;组织培养;鳞片

外文关键词：Lilium asiatic Hybrids Golden Horn; Golden Horn; Tissue cuhure; Bulbs

中文摘要：[目的]利用组织培养的方法对亚洲百合金色号角种进行快繁研究,以实现亚洲百合的工业化生产。[方法]以亚洲百合金色号角种鳞茎为外植体,在其不同的组织培养阶段采用不同的激素配方,以建立亚洲百合组培快繁体系。[结果]亚洲百合愈伤组织诱导的最佳培养基:MS+NAA 0.1 mg/L+6-BA1 mg/L;愈伤组织中不定芽诱导的最佳培养基为MS+NAA 0.1 mg/L+6-BA 3.0 mg/L;最佳生根培养基为1/2MS培养基。[结论]该研究筛选出亚洲百合金色号角最佳的组培快繁培养基,为亚洲百合金色号角种快速繁殖,实现工业化生产提供了技术支持。

外文摘要：[Objective] The aim was to use tissue culture method for the increased reproduction of Lilium asiatic Hybrids Golden Horn, making the industrialized production of Lilium asiatic Hybrids Golden Horn come true. [Method] Using bulbs of Lilium asiatic Hybrids Golden Horn as explants, observing the culture effect in its different tissue culture stages with different hormone combinations, to build Lilium asiatic Hybrids Golden Horn tissue culture system. [Result] The best induction of Lilium asiatic Hybrids Golden Horn callu was MS＋ NAA 0.1 mg/L＋6-BA 1 mg/L;The best adventitious bud induction medium of Lilium asiatic Hybrids Gohlen Horn bulbs was MS ＋NAA 0.1 mg/L ＋ 6-BA 3.0 mg/L; The best root induction medium was 1/2MS. [Conclusion] The optimal tissue culture mediums for Lilium asiatic Hybrids Golden Horn was obtained in this study, it provided technical support for improving the propagation of Lilium asiatic Hybrids Golden Horn and speeding up its industrial production.