文献类型：期刊文献

英文题名：Phase transition of DNA compaction in confined space: Effects of macromolecular crowding are dominant

作者：Chen, Erkun[1];Fan, Yangtao[2];Zhao, Guangju[1];Mao, Zongliang[1];Zhou, Haiping[3];Liu, Yanhui[1]

机构：[1]Guizhou Univ, Coll Phys, Guiyang 550025, Peoples R China;[2]Xiamen Univ, Pen Tung Sah Inst Micronano Sci & Technol, Collaborat Innovat Ctr Optoelect Semicond & Effic, Xiamen 361005, Peoples R China;[3]Shaoxing Univ, Dept Comp Sci & Engn, Shaoxing 312000, Peoples R China

年份：2020

卷号：29

期号：1

外文期刊名：CHINESE PHYSICS B

收录：SCI-EXPANDED(收录号：WOS:000519024500001)、、EI(收录号：20200808187608)、Scopus(收录号：2-s2.0-85079382462)、WOS

基金：Project supported by the National Natural Science Foundation of China (Grant Nos. 11464004 and 11864006), the State Scholarship Fund, China (Grant No. 20173015) and Guizhou Scientific and Technological Program, China (Grant No. 20185781).

语种：英文

外文关键词：macromolecular crowding; Monte Carlo simulation; DNA compaction; phase transition

外文摘要：With a view of detecting the effects of macromolecular crowding on the phase transition of DNA compaction confined in spherical space, Monte Carlo simulations of DNA compaction in free space, in confined spherical space without crowders and in confined spherical space with crowders were performed separately. The simulation results indicate that macromolecular crowding effects on DNA compaction are dominant over the roles of multivalent counterions. In addition, effects of temperature on the phase transition of DNA compaction have been identified in confined spherical space with different radii. In confined spherical space without crowders, the temperature corresponding to phase transition depends on the radius of the confined spherical space linearly. In contrast, with the addition of crowders to the confined spherical space, effects of temperature on the phase transition of DNA compaction become insignificant, whereas the phase transition at different temperatures strongly depends on the size of crowder, and the critical volume fraction of crowders pertains to the diameter of crowder linearly.