文献类型：期刊文献

英文题名：Enhanced delivery of lenvatinib by hyaluronic acid-polyglycerol-stearate self-assembled micelles to inhibit hepatocellular carcinoma in vitro

作者：Luo, Xiang[1,2];Ao, Sha[1,2];Li, Cong[3];Fang, Likun[2];Chen, Lu[2];Liu, Huijie[2];Li, Jiayu[2];Zhou, Yanyan[1];Yin, Xuguang[1];Wu, Chunlei[2];Xi, Meiyang[2];Zhu, Kewu[1]

机构：[1]Shaoxing Univ, Sch Med, Ctr Drug Delivery Syst Res, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Univ, Zhejiang Engn Res Ctr Fat soluble Vitamin, Shaoxing 312000, Zhejiang, Peoples R China;[3]Liaoning Univ, Sch Pharmaceut Sci, Shenyang 110036, Peoples R China

年份：2023

卷号：86

外文期刊名：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001030494200001)、、WOS

基金：This work was supported by the Zhejiang Province Postdoctoral Science Foundation Funded Project (Grant No. ZJ2021024), General Scientific Research Project of Zhejiang Provincial Department of Education (Grant No. Y202145959), Science and Technology Innovation Activity Plan for College Student in Zhejiang Province & amp; XinMiao Talents Program (Grant No. 2022R432A011).

语种：英文

外文关键词：Hyaluronic acid; CD44; Lenvatinib; Polyglycerol; Micelle; Hepatocellular carcinoma

外文摘要：CD44 protein is a well-known surface marker frequently observed in hepatocellular carcinoma (HCC) cells and plays crucial roles in the metastasis and invasion of the tumor. Hyaluronic acid (HA), an endogenous ligand, has the inherent ability to target and endocytosis into tumor cells via CD44 receptor-mediated internalization. The objective of this research was firstly to synthesize a hyaluronic acid-polyglycerol-stearate conjugate (HA-PG10C18), which was then used to self-assemble lenvatinib (Len)-loaded micelles (HA@Len-M) for improved delivery of Len to HCC. The micelles were developed applying a lyophilization-hydration approach. The physicochemical characteristics, including zeta potential, particle size distribution, encapsulation efficiency, morphology, stability and in vitro release were thoroughly assessed for the micelles. Additionally, in vitro cellular uptake studies demonstrated that HA@Len-M facilitated improved uptake of micelles in H22 tumor cells. Furthermore, an in vitro proliferation study of H22 cells showed that the cytotoxicity of HA@Len-M was stronger than other preparations, likely because of the highly efficient delivery to H22 cells by HA@Len-M. These findings suggest that HA@Len-M may offer a CD44 receptor-mediated drug delivery system for eradicating H22, thus representing a potential strategy for tumor-targeted chemotherapy procedures.