文献类型：期刊文献

英文题名：Synthesis and characterization of ZnS:Mn/ZnS core/shell nanoparticles for tumor targeting and imaging invivo

作者：Yu, Zhangsen[1];Ma, Xiying[2];Yu, Bin[1];Pan, Yuefang[1];Liu, Zhaogang[1]

机构：[1]Shaoxing Univ, Sch Med, Shaoxing 312000, Peoples R China;[2]Suzhou Univ Sci & Technol, Sch Math & Phys, Suzhou, Jiangsu, Peoples R China

年份：2013

卷号：28

期号：2

起止页码：232

外文期刊名：JOURNAL OF BIOMATERIALS APPLICATIONS

收录：SCI-EXPANDED(收录号：WOS:000322589600007)、、EI(收录号：20133316606772)、Scopus(收录号：2-s2.0-84872700374)、WOS

基金：This work was supported in parts by the National Natural Science Foundation of China (No. 60976071) and a scientific project of Shaoxing University (2011LG1018).

语种：英文

外文关键词：ZnS:Mn; ZnS nanoparticles; fluorescence; tumor targeting and imaging; invivo; cytotoxicity

外文摘要：Fluorescence imaging technique has been used for imaging of biological cells and tissues invivo. The Cd-free luminescent quantum dots conjugating with a cancer targeting ligand has been taken as a promising biocompatibility and low cytotoxicity system for targeted cancer imaging. This work reports the synthesis of fluorescent-doped core/shell quantum dots of water-soluble manganese-doped zinc sulfide. Quantum dots of manganese-doped zinc sulfide were prepared by nucleation doping strategy, with 3-mercaptopropionic acid as stabilizer at 90? in aqueous solution. The manganese-doped zinc sulfide nanoparticles exhibit strong orange fluorescence under UV irradiation, resistance to photo-bleaching, and low-cytotoxicity to HeLa cells. The structure and optical properties of nanoparticles were characterized by scanning electron microscope, X-ray diffraction, dynamic light scattering, and photoluminescence emission spectroscopy. Manganese-doped zinc sulfide nanoparticles conjugated with folic acid using 2,2-(ethylenedioxy)-bis-(ethylamine) as the linker. The covalent binding of both 2,2-(ethylenedioxy)-bis-(ethylamine) and folic acid on the surface of manganese-doped zinc sulfide nanoparticles probed by Fourier transform infrared spectroscopy detection. Furthermore, invitro cytotoxicity assessment of manganese-doped zinc sulfide-folic acid probes use HeLa cells. The obtained fluorescent probes (manganese-doped zinc sulfide) were used for tumor targeting and imaging invivo. The manganese-doped zinc sulfide-folic acid fluorescent probes which targeting the tumor cells in the body of nude mouse tumor model would emit orange fluorescence, when exposed to a 365nm lamp. We investigate the biodistribution of the manganese-doped zinc sulfide-folic acid fluorescent probes in tumor mouse model by measuring zinc concentration in tissues. These studies demonstrate the practicality of manganese-doped zinc sulfide-folic acid fluorescent probes as promising platform for tumor targeting and imaging invivo.