文献类型：期刊文献

英文题名：Integrating peroxidase-mimicking NH2-MIL-101(Fe) with molecular imprinting for high-performance ratiometric fluorescence sensing of domoic acid

作者：Wang, Linjie[1];Wen, Lejuan[1];Zheng, Shujun[1];Tao, Feifei[3];Chao, Jie[4];Wang, Fei[1,2];Li, Caolong[1,2]

机构：[1]China Pharmaceut Univ, Sch Sci, Dept Chem, Nanjing 211198, Jiangsu, Peoples R China;[2]China Pharmaceut Univ, Cell & Biomol Recognit Res Ctr, Sch Sci, Nanjing 211198, Peoples R China;[3]Shaoxing Univ, Dept Chem & Chem Engn, Shaoxing 312000, Peoples R China;[4]Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn, Minist Educ, Nanjing, Peoples R China

年份：2022

卷号：361

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：SCI-EXPANDED(收录号：WOS:000782121600003)、、WOS

基金：Acknowledgements The authors thank the financial support from the National Key Research and Development Program (2019YFC0312602) as well as the Jiangsu Overseas Visiting Scholar Program for University Prominent Young & Middle-aged Teachers and Presidents. The authors also appreciate the Natural Science Foundation of China (51372154) .

语种：英文

外文关键词：Domoic acid; Ratiometric fluorescent sensor; Peroxidase-like activity; Inner filter effect; Molecular imprinting technology

外文摘要：Ingestion of shellfish and water containing excessive domoic acid (DA) threatens human health tremendously, and even causes death in severe cases. Herein, we have integrated the bifunctional NH2-MIL-101(Fe) with molecular imprinting to design a ratiometric fluorescent sensor for quantitative analysis of DA. In this research, NH2-MIL-101(Fe) itself can supply an intrinsic peak at 452 nm, and trigger oxidation of o-phenylenediamine (OPD) to 2,3-diaminophenazine (DAP) with peak at 556 nm owing to its excellent peroxidase-like activity. The two signal probes that influence each other owing to the inner filter effect (IFE) can form a highly sensitive composite fluorescent probe. Additionally, the introduction of molecular imprinting technology (MIT) instead of expensive antibodies can endow the ratiometric fluorescent sensor outstanding selectivity and specificity toward DA. Thus, the proposed ratiometric fluorescent sensor exhibits outstanding sensitivity, super anti-interference ability, and excellent selectivity. The experimental results demonstrate that the sensor exhibits good performance for DA sensing in the scope of 0.01-10 mu M with low limit of detection (LOD) as 8.2 nM. More attractively, the successful spike and recovery experiments in real samples with the complicated matrix indicate the considerable practical application promise of our proposed ratiometric fluorescent sensor.