文献类型：期刊文献

中文题名：HBsAg携带患儿白介素-10、白介素-12及γ干扰素与HBV DNA的相互关系及意义

英文题名：Study of serum levels of interleukin-10,interleukin-12,interferon-γ and HBV DNA in HBsAg carrier children

作者：张雷家[1];张金萍[2];刘春玲[3]

机构：[1]绍兴文理学院医学院附属医院儿科;[2]绍兴文理学院医学院;[3]山东济宁传染病医院

年份：2006

卷号：29

期号：2

起止页码：92

中文期刊名：上海医学

外文期刊名：Shanghai Medical Journal

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

语种：中文

中文关键词：儿童;肝炎病毒;乙型;细胞因子;HBV;DNA

外文关键词：Children; Hepatitis B virus; Cytokines; HBV DNA

中文摘要：目的探讨HBsAg携带患儿细胞因子白介素(IL)-10I、L-12和γ干扰素(IFN-γ)血清水平的变化及其与HBV DNA的相互关系和意义。方法49例HBsAg携带患儿和30例健康儿童(对照组)均于清晨空腹采血,以双抗夹心酶联免疫吸附试验(ELISA)检测IL-10I、L-12和IFN-γ血清水平,并同时采用荧光定量聚合酶链反应(PCR)检测HBV DNA水平。结果HBsAg携带患儿的IL-10I、L-12和IFN-γ水平均显著高于对照组(P值分别<0.05、<0.01和<0.01)。高病毒载量亚组IL-10和IFN-γ水平均显著高于对照组(P值均<0.01),也明显高于低病毒载量亚组(P值分别<0.01和<0.05),而IL-12则无明显变化。IL-10与IL-12和IFN-γ呈正相关(r值分别=0.4882和0.8712,P<0.01)。IL-12与IFN-γ呈正相关(r=0.5376,P<0.01)。结论HBsAg携带患儿存在异常的细胞免疫应答,IL-10I、L-12和IFN-γ均参与小儿HBV感染的过程,并且与HBV DNA水平密切相关。

外文摘要：Objective To appraise the relevance of serum interleukin （IL） -10, IL- 12, interferon （IFN）-γ levels and HBV DNA in HBsAg carrier children. Methods The serum levels of IL-10, IL-12, IFN-γ and HBV DNA of 49 HBsAg carrier were measured by specific-ELISA and PCR respectively with 30 healthy children as the controls. Results The serum levels of IL-10, IL-12, IFN-γ in HBsAg carrier children were significantly higher than those in the controls （P〈0.05, 〈0.01, 〈0.01 respectively）. The serum levels of IL-10, IFN-γ of high virus-load group were significantly higher than those in the controls （P〈0.01） and the low load group （P〈0.01, 〈0.05 respectively）. IL-10 level was positively correlated with serum levels of IL-12 and IFN-γ （r= 0. 4882,0. 8712, P〈0.01, respectively）, serum IL-12 was positively correlated with the IFN-γ levels （r= 0. 5376, P〈0.01）. Conclusions There exists an abnormal cell mediated immune response in HBsAg carrier children. IL-10, IL-12 and IFN-γ are all involved in the infectious process of children with hepatitis B virus infec tion and are closely correlated with the HBV DNA level. （Shanghai Med J, 2006, 29：92-94）