文献类型：期刊文献

英文题名：Screening and Identification of Linear B Cell Epitopes Within the Nonstructural Proteins of Enterovirus 71

作者：Zhang, Jianhua[1];Huang, Huiqi[1];Xu, Lian[1];Lou, Chaonan[1];Pan, Mi[1]

机构：[1]Shaoxing Univ, Sch Med, Dept Lab Med, 900 Chengnan Ave, Shaoxing 312000, Zhejiang, Peoples R China

年份：2019

卷号：32

期号：2

起止页码：84

外文期刊名：VIRAL IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000452604000001)、、Scopus(收录号：2-s2.0-85062643962)、WOS

基金：The authors are grateful to Dr. Longfei Mao (center for disease control and prevention of Yuncheng district, Shaoxing city) for his help in collecting healthy children sera samples. This work was supported by grants from the Natural Science Foundation of Zhejiang Province (grant no. LY16H190005).

语种：英文

外文关键词：enterovirus 71; hand-foot-and-mouth disease; B cell epitope mapping; nonstructural proteins

外文摘要：Enterovirus 71 (EV-A71) has attracted widespread attention in the past decade because of its higher neurotropicity in addition to causing hand-foot-and-mouth disease (HFMD). Mapping epitopes of viral proteins may promote our understanding of antiviral humoral immunity, and is useful for clinical application. In this study, the linear B cell epitopes within nonstructural proteins of EV-A71 were identified using bioinformatics methods combined with peptide enzyme-linked immunosorbent assay (ELISA). Twenty epitopes were predicted and located at 2ABC (7), 3ABC (5), and 3D (8) protein, respectively. Of 20 epitope-containing peptides, 14 were verified by ELISA (S/CO >2.1), 9 of which had higher reactivity (S/CO >5.0). Furthermore, synthetic peptide SP09 (EV-A71-3ABC(26-41)) can react with healthy children sera, and its immunoreactivity was closest to that of EV-A71-VP1 protein. The protein BLAST analysis revealed that SP09 may contain a common epitope due to the high homology of amino acid sequences with other members of human Enterovirus species A. These findings may be useful in the development serological tests for the diagnosis of HFMD caused by a broad range of human Enterovirus species A.