文献类型：期刊文献

中文题名：微管相关蛋白2和巢蛋白在人胚胎脊髓的表达

英文题名：Expression of microtubule-associated protein 2 and nestin in the developing spinal cord of the human embryo

作者：张泳[1];刘学红[1]

机构：[1]绍兴文理学院医学院

年份：2011

卷号：42

期号：6

起止页码：832

中文期刊名：解剖学报

外文期刊名：Acta Anatomica Sinica

收录：CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：绍兴市科技计划资助项目(2009A33025)

语种：中文

中文关键词：脊髓;微管相关蛋白2;巢蛋白;免疫组织化学;人胚胎

外文关键词：Spinal cord ; Mierotubule-associated protein 2 ; Nestin ; Immunohistochemistry ; Human embryo

中文摘要：目的探讨微管相关蛋白2(MAP-2)和巢蛋白在人胚胎脊髓发育阶段的分布规律及其表达意义。方法应用免疫组织化学法,检测第2、3、4月龄段共16例人胚胎脊髓前角、中央管及后角中MAP-2和巢蛋白的表达、分布状况。结果第2～4个月龄段,人胚胎脊髓内均可见巢蛋白表达阳性的神经纤维分布,随着胎龄的增大,脊髓前角处巢蛋白阳性表达的神经前体细胞和神经胶质细胞呈先增多再降低的趋势,在后角呈逐渐增高趋势。MAP-2在脊髓前后角神经细胞胞质中呈阳性表达,随胎龄增大而增强。结论 MAP-2和巢蛋白参与调控人胚胎脊髓神经细胞和神经胶质细胞的生长发育。

外文摘要：Objective To investigate distribution of the microtubule-associated protein 2 （MAP-2） and nestin in the human developing embryonic spinal cord. Methods Sixteen human embryos, ranged from 2 to 4 months old were used in the present study. Immunohistochemical staining was uesd to detect the expression and distribution of MAP-2 and nestin in the anterior horn, central canal and posterior horn of the spinal cord. Results At the stages of the second to fourth months, nestin-positive nerve fibers were seen within the human embryonic spinal cord, with increase of the gestational age, the expression of nestin-positive neural precursor cells and glial cells first increased and then decreased in the anterior horn, but that in the posterior horn gradually increased. MAP-2 in the anterior and posterior horns of spinal cord nerve cells were positive, with increasing gestational age. Conclusion MAP-2 and nestin involve in the regulation of spinal cord neurons and glial cells during the human embryonic growth and development.