文献类型：期刊文献

英文题名：Ovalbumin-loaded paramagnetic nano-triangles for enhanced dendritic cell stimulation, T 1-MR imaging, and antitumor immunity

作者：Hou, Yike[1];Tang, Zhe[1];Farheen, Jabeen[1];Saeed, Madiha[2,3];Luo, Lijia[4];Ren, Wenzi[4];Luo, Dandan[1];Mushtaq, Asim[1];Zhao, Ruibo[1,5];Ge, Jian[6];Yu, Zhangsen[7];Li, Yao[1];Iqbal, M. Zubair[1];Kong, Xiangdong[1]

机构：[1]Zhejiang Sci Tech Univ, Inst Smart Biomed Mat, Sch Mat Sci & Engn, Hangzhou 310018, Peoples R China;[2]Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China;[4]Chinese Acad Sci, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, CAS Key Lab Magnet Mat & Devices,Key Lab Addit Mfg, Ningbo 315201, Peoples R China;[5]Imperial Coll London, Dept Mat, London SW7 2AZ, England;[6]China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China;[7]Shaoxing Univ, Med Sci Res Ctr, Sch Med, Lab Nanomed, Shaoxing 312000, Peoples R China

年份：2023

卷号：148

起止页码：123

外文期刊名：JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000920656400001)、、EI(收录号：20230413417090)、Scopus(收录号：2-s2.0-85146439913)、WOS

基金：This work was financially supported by the National Natural Science Foundation of China (No. 51672250 and 51902289) , the Zhejiang International Science and Technology Cooperation Project (Nos. 2021C01180 and 2019C04020) , and the Research Foundation of ZSTU (No. 18012134-Y) .

语种：英文

外文关键词：Ovalbumin; Nanovaccine; Antigen delivery; Magnetic resonance imaging; Contrast agent; Cancer immunotherapy

外文摘要：Vaccine-based cancer immunotherapy has demonstrated a significant potential for cancer treatment in clinics. Although the efficiencies of vaccines are limited, they can be enhanced by a well-designed antigen delivery system that promotes sufficient antigen presentation of dendritic cells (DCs) for initiating high T cell immunity. Herein, antigen-loaded manganese oxide (Mn3O4) triangular-shaped ultrasmall nanoparti-cles were prepared to stimulate DC-based immunotherapy under the guidance of T 1 magnetic resonance imaging. The FDA-approved triblock copolymer Pluronic (R) F-68 was used not only to transfer the phase from hydrophobic to hydrophilic but also to enrich antigen loading and improve the biocompatibility of the prepared nanoparticles. Ovalbumin (OVA), a model antigen, was adsorbed on the surface of polymer-coated nanoparticles through electrostatic interaction to form Mn3O4 @PF68-OVA nanoparticle-antigen complexes to stimulate DC-based immunization and antigen-specific T cell immunity. The Mn3O4 @PF68-OVA nanovaccine (NV) induces negligible toxicity effects against 4T1 and bone marrow-derived dendritic cells (BMDCs) by conventional methods supports the proliferation of intestine organoids, which are an innovative three-dimensional cytotoxicity evaluation system, thereby indicating their potential safety for in vivo cancer therapies. The designed paramagnetic nanovaccine possessed excellent OVA delivery to dendritic-regulated antigen-specific T cells in vitro by stimulating the maturation level of BMDCs. In ad-dition, Mn3O4 @PF68-OVA NVs enhance immunity in vivo by increasing the T-cells and M1 macrophages, which suggests improved immunity. Excitingly, vaccination with Mn3O4 @PF68-OVA offer complete pro-tection in the prophylactic group and significant tumor inhibition in the therapeutic group against B16 -OVA tumor. In addition, the designed nanovaccine demonstrated high T 1-MR imaging in the tumor, fur-ther justifying enhanced tumor accumulation and capability to real-time monitor the treatment proce-dure. This study presents a promising nanosystem to design an effective nanovaccine for T 1-MR imaging-guided tumor immunotherapy.(c) 2023 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.