文献类型：期刊文献

中文题名：神经元特异性烯醇化酶和突触素在人胚胎胃发育阶段的表达

英文题名：Gastric expressions of neuron-specific enolase and synaptophysin in human fetuses of 2 to 4 gestational months

作者：刘学红[1];张泳[1];陈小萍[1];黄丹文[1];俞爱月[1]

机构：[1]绍兴文理学院医学院

年份：2014

卷号：34

期号：8

起止页码：1212

中文期刊名：南方医科大学学报

外文期刊名：Journal of Southern Medical University

收录：MEDLINE(收录号：25176100)、CSTPCD、、北大核心2011、Scopus(收录号：2-s2.0-84940520212)、北大核心、CSCD、PubMed、CSCD2013_2014

基金：浙江省教育厅科研计划项目(Y201018535)

语种：中文

中文关键词：胃;突触素;神经元特异性烯醇化酶;胚胎;免疫组织化学

外文关键词：stomach; synaptophysin; neuron-specific enolase; embryo; immunohistochemistry

中文摘要：目的探讨神经元特异性烯醇化酶（neuron-specific enolase,NSE）和突触素（synaptophysin,SYN）在人胚胎胃发育阶段的分布规律及其表达意义。方法应用免疫组织化学法检测第2~4个月龄段,共16例人胚胎,胃壁组织内SYN和NSE的表达、分布状况。结果第2~4个月胎龄段,NSE在人胚胎胃肌间神经丛内的神经细胞及神经纤维均呈强阳性表达;在粘膜下层,随着胎龄的增大,NSE阳性表达细胞和纤维数量逐渐增多,在黏膜层NSE呈阴性表达。第2个月胎龄段,SYN在人胚胎胃肌间神经丛呈阳性表达,在粘膜层内呈阴性表达;第3~4个月龄段,SYN在胃粘膜层、粘膜下层及肌间神经丛内均有阳性表达,随着胎龄增大,阳性表达数量逐渐增多。结论人胚胎胃发育阶段,NSE和SYN在胃壁的阳性表达和分布不一致,均参与胃壁神经系统的发育过程。

外文摘要：Objective To investigate the distribution patterns of neuron-specific enolase （NSE） and synaptophysin （SYN） during the development of human fetal stomach. Methods Sixteen specimens of human fetal （gestational age 2 to 4 months） gastric tissues were examined with immunohistochemistry for detecting the distribution of NSE and SYN expressions in the gastric walls. Results During the second to fourth gestational months, NSE was strongly expressed in the nerve cells and nerve fibers of the myenteric nerve plexus of human fetal stomach. As the gestational age increased, the numbers of NSE positive cells and fibers increased gradually in the gastric submucosa, but NSE was negative in the gastric mucosa. At the second gestational month, SYN expression was negative in the mucosa but positive in the myenteric nerve plexus; during the third to fourth months, positive SYN expression was found in the mucosa, submucosa and myenteric nerve plexus of the embryonic gastric walls and its expression intensity increased with the gestational age. Conclusion SYN and NSE are both involved in the regulation of the nervous system in the gastric wall but their expressions and distributions follow different patterns during the development of human fetal stomach.