文献类型：期刊文献

英文题名：EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier

作者：Fang, Jian[1,2];Zhang, Zhuangwei[1];Cheng, Yinyin[1];Yang, Haitao[3];Zhang, Hui[1];Xue, Zhe[1];Lu, Songtao[1];Dong, Yichen[1];Song, Chunyan[1];Zhang, Xiaohong[1,4];Zhou, Yuping[4,5]

机构：[1]Ningbo Univ, Sch Med, Dept Prevent Med, Zhejiang Key Lab Pathophysiol, 818 Fenghua Rd, Ningbo 315211, Zhejiang, Peoples R China;[2]Shaoxing Univ, Coll Med, 508 Huancheng Rd, Shaoxing 312000, Zhejiang, Peoples R China;[3]Zhejiang Univ, Dept Pathol, Mingzhou Hosp, Ningbo 315040, Zhejiang, Peoples R China;[4]Ningbo Univ, Med Sch, Dept Gastroenterol & Hepatol, Affiliated Hosp, 247 Renmin Rd, Ningbo 315020, Zhejiang, Peoples R China;[5]Ningbo Univ, Inst Digest Dis, Ningbo 315020, Peoples R China

年份：2022

卷号：13

期号：8

起止页码：4399

外文期刊名：FOOD & FUNCTION

收录：SCI-EXPANDED(收录号：WOS:000770038300001)、、EI(收录号：20221511955473)、Scopus(收录号：2-s2.0-85127895331)、WOS

基金：The authors want to thank Jige Li at the Ningbo Municipal Center for Disease Control and Prevention. We thank Westlake University Laboratory of Human Nutrition and Epidemiology for assistance in the 16S rRNA-based gut microbiota analysis. We thank all participants and staff in the participating studies for their contribution to the study. We would like to thank Editage for English language editing. This study was supported by the National Natural Science Foundation of China (NSFC; Grant No. 81872620, 81673163), Natural Science Foundation of Zhejiang Province (LY21H260001), Zhejiang Key Laboratory of Pathophysiology (201802), Zhejiang Public Welfare Technology Applied Research Project (LGF18H060006) and Zhejiang Provincial Medical and Health Science and Technology plan (2018KY819; 2022KY1311). This study was sponsored by the K. C. Wong Magna Fund in Ningbo University, Shaoxing Municipal Science and Technology Project of Medical and Health (2020A13064), the Scientific Research Projects of Shaoxing University (2019SK003) and the Scientific Research Start-up Project of Shaoxing University (20210019).

语种：英文

外文关键词：Crosstalk - Diseases - Gas chromatography - Mammals - Propionic acid - Reinforcement - RNA - Saturated fatty acids - Sodium sulfate - Sulfur compounds

外文摘要：Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course. Its etiology is closely related to abnormal interactions between host and gut microbiota. The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota. Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy. Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA. Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR. Western blotting was used to analyze the underlying molecular events. Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing. Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation. In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids. Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis. EPA may develop into a promising adjunctive therapy for UC.