文献类型：期刊文献

英文题名：Mitigating N2O emission by synthetic inhibitors mixed with urea and cattle manure application via inhibiting ammonia-oxidizing bacteria, but not archaea, in a calcareous soil

作者：Tao, Rui[1];Li, Jun[1];Hu, Baowei[1];Chu, Guixin[1]

机构：[1]Shaoxing Univ, Sch Life Sci, Shaoxing 312000, Peoples R China

年份：2021

卷号：273

外文期刊名：ENVIRONMENTAL POLLUTION

收录：SCI-EXPANDED(收录号：WOS:000625376600061)、、WOS

基金：Our study was collectively supported by the Basic PublicWelfare Research Project of Zhejiang Province, P. R. China (LGN20C150003), Natural Science Foundation of Shaoxing University, P. R. China (2018LG1003), and Special Fund for Agro-scientific Research in the Public Interest, P. R. China (201503116).

语种：英文

外文关键词：Synthetic inhibitors; Nitrous oxide emissions; Organic manure; Ammonia-oxidizer communities; Temperature

外文摘要：Synthetic inhibitors and organic amendment have been proposed for mitigating greenhouse gas N2O emissions. However, their combined effect on the N2O emissions and ammonia-oxidizer (ammonia-oxidizing bacteria and archaea, AOB and AOA) communities remains unclear in calcareous soils under climate warming. We conducted two incubation experiments (25 and 35 degrees C) to examine how N2O emissions and AOA and AOB communities responded to organic amendment (urea plus cattle manure, UCM), and in combination with urease (N-(n-butyl) thiophosphoric triamide, NBPT) and nitrification inhibitor (nitrapyrin). The treatments of UCM + nitrapyrin and UCM + nitrapyrin + NBPT significantly lowered total N2O emissions by average 64.5 and 71.05% at 25 and 35 degrees C, respectively, compared with UCM treatment. AOB gene abundance and a-diversity (Chao1 and Shannon indices) were significantly increased by the application of urea and manure (P < 0.05). However, relative to UCM treatment, nitrapyrin addition treatments decreased AOB gene abundance and Chao 1 index by average 115.4 and 30.4% at 25 and 35 degrees C, respectively. PCA analysis showed that UCM or UCM plus nitrapyrin notably shifted AOB structure at both temperatures. However, fertilization had little effects on AOA community (P > 0.05). Potential nitrification rate (PNR) was greatly decreased by nitrapyrin addition, and PNR significantly positively correlated with AOB gene abundance (P = 0.0179 at 25 degrees C and P = 0.0029 at 35 degrees C) rather than AOA (P > 0.05). Structural equation model analysis showed that temperature directly increased AOA abundance but decrease AOB abundance, while fertilization indirectly influenced AOB community by altering soil NH4+, pH and SOC. In conclusion, the combined application of organic amendment, NBPT and nitrapyrin significantly lowered N2O emissions via reducing AOB community in calcareous soil even at high temperature. Our findings provide a solid theoretical basis in mitigating N2O emissions from calcareous soil under climate warming. (C) 2021 Elsevier Ltd. All rights reserved.