文献类型：期刊文献

英文题名：Development of a Specific Latex Agglutination Test to Detect Antibodies of Enterovirus 71

作者：Qin, Bo[1,2];Zhang, Jianhua[3];Xie, Wenhao[4];Liu, Xuehong[3];He, Tingting[2];Chen, Jinkun[2];Dong, Xuejun[1]

机构：[1]Zhejiang Univ, Shaoxing Hosp, Shaoxing Peoples Hosp, Shaoxing 312000, Zhejiang, Peoples R China;[2]Shaoxing Ctr Dis Control & Prevent, Shaoxing, Zhejiang, Peoples R China;[3]Shaoxing Univ, Coll Med, Shaoxing, Peoples R China;[4]Inst Appl Nephrol Interneph, DE-52066 Aachen, Germany

年份：2015

卷号：46

期号：7

起止页码：580

外文期刊名：ARCHIVES OF MEDICAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000366145800010)、、Scopus(收录号：2-s2.0-84946027322)、WOS

基金：We are grateful to the patients for providing the serum samples. This work was supported by Science Technology Bureau of Shaoxing, Zhejiang Province of China (2013B70063 and 2012B70063) and Zhejiang Medical Technology and Education program (2013KYA208).

语种：英文

外文关键词：Enterovirus 71; Hand-foot-and-mouth disease; Latex agglutination test; Homogeneity; Ethyl-dimethyl-amino-propyl carbodiimide

外文摘要：A latex agglutination test (LAT) was developed for the rapid detection of antibodies against the VP1 or VP1 proteins of Enterovirus 71 (EV71). The proteins of interest including prokaryotically expressed VP1 and two strains of anti-VP1 monoclonal antibody (McAb) against EV71 were covalently linked to carboxylated latex using ethyldimethyl-amino-propyl carbodiimide (EDC), to prepare sensitized latex beads. LAT was evaluated by an enzyme-linked immunosorbent assay (ELISA) as a reference test. The VP1-LAT showed a sensitivity of 87.0%, specificity of 88.9%, and an agreement ratio of 90.0% in detecting VP1 in 100 serum samples from experimentally infected mice, whereas these values were 86.8, 96.7, and 93.3%, respectively, for 608 clinical human serum samples. The VP1-LAT has advantages over other assays in terms of low cost, rapidity, chemical stability, high sensitivity, repeatability, and specificity. The LAT established in the present study is a rapid and simple test suitable for field monitoring of antibodies against VP1-EV71. (C) 2015 IMSS. Published by Elsevier Inc.