文献类型：期刊文献

中文题名：注射法培育三角帆蚌有核珍珠的研究

英文题名：Study on culturing pearl sacs in vitro and injection method for pearl producing

作者：钱伟平[1];许梓荣[2];张明霞[1];施妙琴[1]

机构：[1]绍兴文理学院生物系;[2]浙江大学动物科学学院

年份：2002

卷号：14

期号：2

起止页码：82

中文期刊名：浙江农业学报

外文期刊名：Acta Agriculturae Zhejiangensis

收录：CSTPCD、、CSCD_E2011_2012、CSCD

语种：中文

中文关键词：注射法;三角帆蚌;有核珍珠;珍珠囊体外培育;细胞悬液;胰蛋白酶

外文关键词：Hyriopsis cumingii; cultured pearl sac in vitro ; pallium cell injection; producing nucleated pearls

中文摘要：对三角帆蚌有核珍珠培育方法作了研究。珍珠囊体外培育采用胰蛋白酶对外套膜组织块进行解离 ,收集细胞悬液于珠核上培养珍珠囊 ,然后将体外形成的有核珍珠囊再进行插核 ,并将酶解处理后的细胞悬液注射到所插核部位。结果表明 ,外套膜组织细胞悬液贴核生长正常 ,并且能够培育出体外珍珠囊 ;将体外珍珠囊插到母蚌外套膜内 ,同时注射外套膜组织细胞悬液 ,经 6 0天养殖后 。

外文摘要：This article focuses on the new technology for producing nucleated pearls using Hyriopsis cumingii Lea's. Pallium tissue was scattered by trypsin solution. Pearl nuclear was treated by pallium tissue piece in order to culture pearl sacs. Cultured pearl sacs in vitro were inserted into pallium tissue for producing pearls. Meanwhile, the pallium cells slurry was injected into the pallium of the Hyriopsis cumingii Lea. The result showed that the new methods can induce the formation of pearl sacs. Sixty days later the pearl can be seen on the surface of the nucleate sacs. It is suggested pearl sac induced by injection may be the best method to produce pearl.